Differential cellular responses associated with oxidative stress and cell fate decision under nitrate and phosphate limitations in Thalassiosira pseudonana: Comparative proteomics.
Differential cellular responses associated with oxidative stress and cell fate decision under nitrate and phosphate limitations in Thalassiosira pseudonana: Comparative proteomics.
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假微型海链藻硝酸盐和磷酸盐限制下与氧化应激和细胞命运决定相关的差异细胞反应：比较蛋白质组学
DOI：
10.1371/journal.pone.0184849
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发表时间：
2017
期刊：
影响因子：
3.7
通讯作者：
Gao YH
中科院分区：
文献类型：
--
作者：
Lin Q;Liang JR;Huang QQ;Luo CS;Anderson DM;Bowler C;Chen CP;Li XS;Gao YH
硅藻是海洋生态系统的重要组成部分，对世界初级生产做出了巨大贡献。尽管硅藻在生态系统中发挥着重要作用，但硅藻如何科普营养波动引起的氧化应激的分子基础在很大程度上仍然未知。本研究采用iTRAQ蛋白质组学方法，结合一系列生理生化技术，探讨了氮和磷胁迫对海链藻氧化应激和细胞命运决定相关的细胞和代谢的影响。共检测到1151个蛋白质，122个和56个显着差异表达的控制下，N-和P-限制条件下，分别。在氮限制的细胞中，响应蛋白与活性氧（ROS）积累，氧化应激反应和细胞死亡，对应于光合效率的显着降低，显着的细胞内ROS积累，半胱天冬酶介导的程序性细胞死亡激活。这些反应都没有被确定在P-有限的细胞，但是，碱性磷酸酶蛋白的显着上调观察到，这可能是P-有限的细胞，以科普环境P缺乏症的主要贡献者。这些发现表明，硅藻在应对不同的营养胁迫和保持细胞活力时，采用了根本不同的代谢反应和细胞调节。
Diatoms are important components of marine ecosystems and contribute greatly to the world’s primary production. Despite their important roles in ecosystems, the molecular basis of how diatoms cope with oxidative stress caused by nutrient fluctuations remains largely unknown. Here, an isobaric tags for relative and absolute quantitation (iTRAQ) proteomic method was coupled with a series of physiological and biochemical techniques to explore oxidative stress- and cell fate decision-related cellular and metabolic responses of the diatom Thalassiosira pseudonana to nitrate (N) and inorganic phosphate (P) stresses. A total of 1151 proteins were detected; 122 and 56 were significantly differentially expressed from control under N- and P-limited conditions, respectively. In N-limited cells, responsive proteins were related to reactive oxygen species (ROS) accumulation, oxidative stress responses and cell death, corresponding to a significant decrease in photosynthetic efficiency, marked intracellular ROS accumulation, and caspase-mediated programmed cell death activation. None of these responses were identified in P-limited cells; however, a significant up-regulation of alkaline phosphatase proteins was observed, which could be the major contributor for P-limited cells to cope with ambient P deficiency. These findings demonstrate that fundamentally different metabolic responses and cellular regulations are employed by the diatom in response to different nutrient stresses and to keep the cells viable.
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DOI：
10.1016/j.bbrc.2007.07.072
发表时间：
2007-09-28
影响因子：
3.1
作者：
Ding, Di;Sagher, Daphna;Zhang, Xing-Hal
通讯作者：
Zhang, Xing-Hal
影响因子：
2.7
作者：
GAGE, MA;GORHAM, E
通讯作者：
GORHAM, E
影响因子：
5.6
作者：
Cormann KU;Möller M;Nowaczyk MM
通讯作者：
Nowaczyk MM
影响因子：
3.7
作者：
Dyhrman ST;Jenkins BD;Rynearson TA;Saito MA;Mercier ML;Alexander H;Whitney LP;Drzewianowski A;Bulygin VV;Bertrand EM;Wu Z;Benitez-Nelson C;Heithoff A
通讯作者：
Heithoff A
影响因子：
4.5
作者：
Berman-Frank, I;Cullen, JT;Falkowski, PG
通讯作者：
Falkowski, PG