The transcriptome and proteome of the diatom Thalassiosira pseudonana reveal a diverse phosphorus stress response.
The transcriptome and proteome of the diatom Thalassiosira pseudonana reveal a diverse phosphorus stress response.
复制标题
DOI：
10.1371/journal.pone.0033768
复制
发表时间：
2012
期刊：
影响因子：
3.7
通讯作者：
Heithoff A
中科院分区：
文献类型：
--
作者：
Dyhrman ST;Jenkins BD;Rynearson TA;Saito MA;Mercier ML;Alexander H;Whitney LP;Drzewianowski A;Bulygin VV;Bertrand EM;Wu Z;Benitez-Nelson C;Heithoff A
磷(P)是海洋浮游植物生长和生态系统功能的关键驱动力。硅藻是丰富的一类海洋浮游植物，负责大量的初级生产。由于它们对海洋碳循环的控制，已经有许多研究集中在硅藻如何应对限制的宏量和微量营养物质，如铁和氮。然而，硅藻对缺磷的生理反应尚不清楚。在这里，我们结合转录标记的深度测序和定量蛋白质组学来分析生长在富磷和缺磷条件下的假海硅藻。共有318个转录本存在差异调控（错误发现率<0.05），136个蛋白存在差异丰度（p<0.05）。转录本和蛋白质丰度的显著变化被观察到，并协调了多种生化途径，包括糖酵解和翻译。转录模式和蛋白质丰度也与细胞P分布和酶活性的生理变化有关。这些数据表明，硅藻P缺乏导致细胞P分配的变化，通过产生多磷酸盐，增加P运输，通过增加金属酶的产生转向利用溶解的有机P，以及通过产生亚脂重塑细胞表面。总之，这些发现揭示了假单胞菌对磷缺乏的复杂反应涉及多种生化策略，这些策略可能对其应对环境磷有效性变化的能力至关重要。
Phosphorus (P) is a critical driver of phytoplankton growth and ecosystem function in the ocean. Diatoms are an abundant class of marine phytoplankton that are responsible for significant amounts of primary production. With the control they exert on the oceanic carbon cycle, there have been a number of studies focused on how diatoms respond to limiting macro and micronutrients such as iron and nitrogen. However, diatom physiological responses to P deficiency are poorly understood. Here, we couple deep sequencing of transcript tags and quantitative proteomics to analyze the diatom Thalassiosira pseudonana grown under P-replete and P-deficient conditions. A total of 318 transcripts were differentially regulated with a false discovery rate of <0.05, and a total of 136 proteins were differentially abundant (p<0.05). Significant changes in the abundance of transcripts and proteins were observed and coordinated for multiple biochemical pathways, including glycolysis and translation. Patterns in transcript and protein abundance were also linked to physiological changes in cellular P distributions, and enzyme activities. These data demonstrate that diatom P deficiency results in changes in cellular P allocation through polyphosphate production, increased P transport, a switch to utilization of dissolved organic P through increased production of metalloenzymes, and a remodeling of the cell surface through production of sulfolipids. Together, these findings reveal that T. pseudonana has evolved a sophisticated response to P deficiency involving multiple biochemical strategies that are likely critical to its ability to respond to variations in environmental P availability.
登录
查看更多内容
影响因子：
3.3
作者：
Le Bihan, Thierry;Martin, Sarah F.;Millar, Andrew J.
通讯作者：
Millar, Andrew J.
影响因子：
56.9
作者：
Armbrust, EV;Berges, JA;Rokhsar, DS
通讯作者：
Rokhsar, DS
DOI：
10.1016/j.bbrc.2005.11.144
发表时间：
2006-02-03
影响因子：
3.1
作者：
Li, QY;Gao, XS;Xu, ZK
通讯作者：
Xu, ZK
影响因子：
56.9
作者：
Diaz, Julia;Ingall, Ellery;Brandes, Jay A.
通讯作者：
Brandes, Jay A.
影响因子：
56.9
作者：
Hothorn, Michael;Neumann, Heinz;Mayer, Andreas
通讯作者：
Mayer, Andreas