Diabetic dyslipidemia and exercise alter the plasma low-density lipoproteome in Yucatan pigs.
Diabetic dyslipidemia and exercise alter the plasma low-density lipoproteome in Yucatan pigs.
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DOI：
10.1002/pmic.200800613
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发表时间：
2009-05
期刊：
影响因子：
3.4
通讯作者：
Witzmann, Frank A.
中科院分区：
文献类型：
--
作者：
Richardson, Matthew R.;Lai, Xianyin;Dixon, Joseph L.;Sturek, Michael;Witzmann, Frank A.
关键词：
虽然低密度脂蛋白（LDL）在动脉粥样硬化形成中起着主要作用，但低密度脂蛋白组尚未完全表征。此外，来自西方饮食、糖尿病和身体不活动对这种蛋白质组的改变还有待确定。因此，与健康对照（C）和非糖尿病高脂血症（H）猪相比，测定了动脉粥样硬化早期阶段雄性尤卡坦糖尿病血脂异常（DD）猪的LDL蛋白的相对定量。重要的是，在这些动物中通过有氧运动训练（DDX）预防冠状动脉血管功能障碍，而不改变总LDL浓度。使用2-DE，蛋白质印迹，无标记定量MS，和选择性反应监测，载脂蛋白A-I，B，C-III，D，E和J和非共价结合蛋白的丰度的变化进行了测定，使用快速蛋白液相色谱分离的LDL。至少有28个独特的蛋白质，其中许多是新的，被确定为高置信度。载脂蛋白E亚型与疾病（内膜增厚的冠状动脉节段百分比）的相关性强于一些传统的风险因素（总胆固醇、LDL胆固醇和LDL/HDL胆固醇）。总之，这项工作确定了新的可能的生物标志物，动脉粥样硬化的潜在治疗靶点，并产生了关于LDL在动脉粥样硬化形成中的作用的新假设。
Although low-density lipoprotein (LDL) plays a predominant role in atherogenesis, the low-density lipoproteome has not been fully characterized. Moreover, alterations from a Western diet, diabetes, and physical inactivity on this proteome have yet to be determined. Accordingly, relative quantification was determined in LDL proteins from male Yucatan diabetic dyslipidemic (DD) swine in the early stages of atherosclerosis compared to healthy control (C) and non-diabetic hyperlipidemic (H) swine. Importantly, coronary vascular dysfunction was prevented by aerobic exercise training in these animals (DDX) without altering total LDL concentration. Using 2-DE, Western blot, label-free quantitative MS, and selected reaction monitoring, alterations in the abundance of apolipoproteins A-I, B, C-III, D, E, and J and noncovalently associated proteins were determined in LDL isolated using fast protein liquid chromatography. At least 28 unique proteins, many of which were novel, were identified with high confidence. An apolipoprotein E isoform demonstrated stronger correlation to disease (percent of coronary artery segments with intimal thickening) than some traditional risk factors (total cholesterol, LDL cholesterol, and LDL/HDL cholesterol). Taken together, this work identifies new possible biomarkers, potential therapeutic targets for atherosclerosis, and generates new hypotheses regarding the role of LDL in atherogenesis.
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影响因子：
56.9
作者：
MAHLEY, RW
通讯作者：
MAHLEY, RW
影响因子：
6.5
作者：
Cohn, JS;Rodriguez, C;Davignon, A
通讯作者：
Davignon, A
影响因子：
6.5
作者：
Dixon, JL;Shen, SM;Sturek, M
通讯作者：
Sturek, M
影响因子：
120.7
作者：
Blair, SN;Kampert, JB;Gibbons, LW
通讯作者：
Gibbons, LW
DOI：
10.1046/j.1526-0968.1999.00157.x
发表时间：
1999-08-01
期刊：
Therapeutic apheresis : official journal of the International Society for Apheresis and the Japanese Society for Apheresis
影响因子：
--
作者：
Geiss, H C;Parhofer, K G;Schwandt, P
通讯作者：
Schwandt, P