Characterization of murine pituitary-derived cell lines Tpit/F1, Tpit/E and TtT/GF.
Characterization of murine pituitary-derived cell lines Tpit/F1, Tpit/E and TtT/GF.
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DOI：
10.1262/jrd.2014-031
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发表时间：
2014
期刊：
影响因子：
--
通讯作者：
Kato Y
中科院分区：
文献类型：
--
作者：
Yoshida S;Higuchi M;Ueharu H;Nishimura N;Tsuda M;Yako H;Chen M;Mitsuishi H;Sano Y;Kato T;Kato Y
脑下垂体是脊椎动物重要的内分泌组织，产生和分泌多种激素。越来越多的数据表明，几种类型的细胞组成垂体，有越来越多的兴趣来阐明这些细胞类型的起源及其在垂体器官发生中的作用。其中，组织细胞系是研究衍生组织功能的极有价值的实验工具。在这项研究中，我们通过微阵列分析和实时PCR比较了小鼠垂体肿瘤来源的非激素产生细胞系TtT/GF、Tpit/F1和Tpit/E的基因表达谱。TtT/GF中有几个特征性表达的基因：Abcg2、Nestin、Prrx1、Prrx2、CD34、Eng、Cspg4 （Ng2）、S100β和nNos；Tpit/F1中的Cxcl12， Raldh1， Msx1和Twist1；Tpit/E中的Cxadr、Sox9、Cdh1、EpCAM和Krt8。TtT/GF细胞表现出分化程度最高的状态，可能具有垂体血管内皮细胞和/或周细胞的某些特性。Tpit/F1细胞显示上皮和间充质表型，干性仍处于过渡状态。Tpit/E细胞具有上皮细胞的表型，是分化过程中或初始内皮-间质转化（EMT）中最不成熟的细胞。因此，这三种细胞系必须成为研究垂体干/祖细胞以及器官发生的有用模型细胞系。
The pituitary is an important endocrine tissue of the vertebrate that produces and secretes many hormones. Accumulating data suggest that several types of cells compose the pituitary, and there is growing interest in elucidating the origin of these cell types and their roles in pituitary organogenesis. Therein, the histogenous cell line is an extremely valuable experimental tool for investigating the function of derived tissue. In this study, we compared gene expression profiles by microarray analysis and real-time PCR for murine pituitary tumor-derived non-hormone-producing cell lines TtT/GF, Tpit/F1 and Tpit/E. Several genes are characteristically expressed in each cell line: Abcg2, Nestin, Prrx1, Prrx2, CD34, Eng, Cspg4 (Ng2), S100β and nNos in TtT/GF; Cxcl12, Raldh1, Msx1 and Twist1 in Tpit/F1; and Cxadr, Sox9, Cdh1, EpCAM and Krt8 in Tpit/E. Ultimately, we came to the following conclusions: TtT/GF cells show the most differentiated state, and may have some properties of the pituitary vascular endothelial cell and/or pericyte. Tpit/F1 cells show the epithelial and mesenchymal phenotypes with stemness still in a transiting state. Tpit/E cells have a phenotype of epithelial cells and are the most immature cells in the progression of differentiation or in the initial endothelial-mesenchymal transition (EMT). Thus, these three cell lines must be useful model cell lines for investigating pituitary stem/progenitor cells as well as organogenesis.
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