Assessment of Drought Tolerance Based Impacts with Over-expression of ZmLTP3 in Maize (Zea mays L.)

Assessment of Drought Tolerance Based Impacts with Over-expression of ZmLTP3 in Maize (Zea mays L.)
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评估玉米 (Zea mays L.) 中 ZmLTP3 过表达对耐旱性的影响

DOI:
10.1556/0806.46.2018.062
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发表时间:
2019-02
影响因子:
1.6
通讯作者:
H. Zou
H. Zou
中科院分区:
农林科学4区
文献类型:
--
作者:
Y. Li;K. Zhou;M. Jiang;B. Zhang;M. Aslam;H. Zou

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大量研究表明,脂转移蛋白(lipid transfer proteins,LTP)在植物的花、发育、表皮蜡质沉积和病原菌应答等过程中发挥重要作用,但其在非生物胁迫中的作用报道相对较少。本研究对玉米LTP基因(ZmLTP3)在干旱胁迫下的功能进行了研究。将ZmLTP3基因导入玉米自交系京2416,对转基因玉米自交系进行草甘膦抗性和耐旱性分析。分析表明,转基因系能显著提高耐旱性。转基因玉米OE6、OE7和OE8在干旱条件下表现出较低的细胞膜损伤、较高的叶绿素含量、较高的保护酶活性和较好的生长发育。结果强烈表明,ZmLTP3的过表达可以提高玉米的耐旱性。
Numerous studies showed that lipid transfer proteins (LTPs) play important roles in flower, development, cuticular wax deposition and pathogen responses; however, their roles in abiotic stresses are relatively less reported. This study characterized the function of a maize LTP gene (ZmLTP3) during drought stress. ZmLTP3 gene was transferred into maize inbred line Jing 2416; subsequently the glyphosate and drought tolerance of the over-expression (OE) lines were analyzed. Analysis showed that OE lines could significantly enhance drought tolerance. Transgenic maize lines OE6, OE7 and OE8 showed lower cell membrane damage, higher chlorophyll contents, higher protective enzymes activities, better growth and development under drought condition. The results strongly indicated that overexpression of ZmLTP3 could increase drought tolerances in maize..
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