Coordinate regulation of carbohydrate metabolism and virulence by PtsH in pathogen Edwardsiella piscicida.
Coordinate regulation of carbohydrate metabolism and virulence by PtsH in pathogen Edwardsiella piscicida.
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DOI：
10.1007/s00253-022-11848-8
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发表时间：
2022-03
影响因子：
5
通讯作者：
Wang Q
中科院分区：
文献类型：
--
作者：
Mao Q;Jiang J;Wu X;Xu R;Ma Y;Zhang Y;Shao S;Wang Q
细菌病原体的碳水化合物代谢在致病过程中起着重要的调控作用，但其代谢和毒力相互调控的分子机制仍有待阐明。在这里，我们调查了在这方面，杀鱼爱德华氏菌，一个臭名昭著的人畜共患病原体以前被命名为E。能发酵包括葡萄糖、果糖、甘露糖、N-乙酰葡糖胺和N-乙酰半乳糖胺在内的很少PTS糖的tarda。我们系统地描述了预测的23个磷酸转移酶系统（PTS）的组成部分中的每一个的作用与相应的框内缺失突变体和含有特定PTS糖的限定培养基。此外，PtsH被确定为关键的PTS组分，增强了所有测试的PTS糖的利用。有趣的是，我们还发现PtsH而不是Fpr参与了T3 SS基因的表达，并且在E.杀鱼的为了证实这一点，His 15和Ser 46，两个已建立的PtsH残基参与磷酸化级联反应，在调节T3 SS产量显示冗余的作用。此外，PtsH显示促进甘露糖摄取并将其转化为甘露糖-6-磷酸，甘露糖-6-磷酸是一种建立用于激活EvrA以增强细菌毒力的变构底物。总的来说，我们的观察提供了新的见解PTS β调节碳水化合物代谢和毒力基因表达的作用。 ·系统地确定了杀鱼爱德华氏菌PTS组分对糖吸收的作用。 · PtsH参与E. coli的摄取和调节。杀鱼素的T3 SS表达。 ·His 15和Ser 46处的PtsH磷酸化对于T3 SS表达和毒力是必需的。在线版本包含补充材料，可通过10.1007/s 00253 -022-11848-8获得。
Carbohydrate metabolism of bacterial pathogens conducts crucial roles in regulating pathogenesis but the molecular mechanisms by which metabolisms and virulence are been modulated and coordinated remain to be illuminated. Here, we investigated in this regard Edwardsiella piscicida, a notorious zoonotic pathogen previously named E. tarda that could ferment very few PTS sugars including glucose, fructose, mannose, N-acetylglucosamine, and N-acetylgalactosamine. We systematically characterized the roles of each of the predicted 23 components of phosphotransferase system (PTS) with the respective in-frame deletion mutants and defined medium containing specific PTS sugar. In addition, PtsH was identified as the crucial PTS component potentiating the utilization of all the tested PTS sugars. Intriguingly, we also found that PtsH while not Fpr was involved in T3SS gene expression and was essential for the pathogenesis of E. piscicida. To corroborate this, His15 and Ser46, the two established PtsH residues involved in phosphorylation cascade, showed redundant roles in regulating T3SS yields. Moreover, PtsH was shown to facilitate mannose uptake and transform it into mannose-6-phosphate, an allosteric substrate established to activate EvrA to augment bacterial virulence. Collectively, our observations provide new insights into the roles of PTS reciprocally regulating carbohydrate metabolism and virulence gene expression. • PTS components’ roles for sugar uptake are systematically determined in Edwardsiella piscicida. • PtsH is involved in saccharides uptake and in the regulation of E. piscicida’s T3SS expression. • PtsH phosphorylation at His15 and Ser46 is essential for the T3SS expression and virulence. The online version contains supplementary material available at 10.1007/s00253-022-11848-8.
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影响因子：
5.2
作者：
通讯作者：
--
影响因子：
4.4
作者：
Gao, Zhi Peng;Nie, Pin;Xie, Hai Xia
通讯作者：
Xie, Hai Xia
影响因子：
8.8
作者：
Maze, Alain;Glatter, Timo;Bumann, Dirk
通讯作者：
Bumann, Dirk
DOI：
10.1007/0-387-30746-x_4
发表时间：
2006-01-01
期刊：
PROKARYOTES: A HANDBOOK ON THE BIOLOGY OF BACTERIA, VOL 6, THIRD EDITION
影响因子：
--
作者：
Abbott, Sharon L.;Janda, J. Michael
通讯作者：
Janda, J. Michael
影响因子：
3.6
作者：
Sundar, Ganesh S.;Islam, Emrul;McIver, Kevin S.
通讯作者：
McIver, Kevin S.