Two-dimensional intravascular near-infrared fluorescence molecular imaging of inflammation in atherosclerosis and stent-induced vascular injury.
Two-dimensional intravascular near-infrared fluorescence molecular imaging of inflammation in atherosclerosis and stent-induced vascular injury.
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DOI：
10.1016/j.jacc.2011.02.036
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发表时间：
2011-06-21
影响因子：
24
通讯作者：
Ntziachristos, Vasilis
中科院分区：
文献类型：
--
作者：
Jaffer, Farouc A.;Calfon, Marcella A.;Rosenthal, Amir;Mallas, Georgios;Razansky, R. Nika;Mauskapf, Adam;Weissleder, Ralph;Libby, Peter;Ntziachristos, Vasilis
开发二维血管内近红外荧光 (NIRF) 成像策略，用于研究冠状动脉大小的血管中的动脉炎症。动脉炎症的分子成像可以为冠状动脉粥样硬化和植入支架引起的急性心肌梗死的发病机制提供新的见解。目前，很少有高分辨率炎症方法可以对冠状动脉大小的动脉炎症进行体内成像。新型 2.9F 旋转、自动回拉二维成像导管专为 360 度观察血管内 NIRF 成像而设计和优化。与半胱氨酸蛋白酶激活成像报告仪 Prosense VM110 结合，对动脉粥样硬化兔主动脉 (n=10) 或植入冠状动脉裸金属支架 (n=10，直径 3.5mm，植入后第 7 天) 进行动脉内 2D NIRF 成像。血管内超声（IVUS）提供了动脉的共同配准解剖图像。处死后，对标本进行离体 NIRF 成像、荧光显微镜以及组织学和免疫组织化学分析。冠状动脉尺度模型的成像显示出 8 扇区角分辨率和亚毫米轴向分辨率、对 NIR 荧光染料的纳摩尔级敏感性以及通过血液的适度 NIRF 光衰减。通过血液实时获得信噪比 > 10 的血管壁炎症高分辨率 NIRF 图像，无冲洗或闭塞。在动脉粥样硬化中，2D NIRF、IVUS-NIRF 融合、显微镜和免疫印迹研究提供了对斑块蛋白酶活性空间分布的深入了解。在植入支架的血管中，实时成像揭示了支架引起的动脉炎症的基于边缘的模式。一种新的 2D 血管内 NIRF 成像策略提供了冠状动脉大小的动脉炎症的高分辨率体内空间图，并揭示了动脉粥样硬化和支架诱导的动脉损伤中炎症调节的半胱氨酸蛋白酶活性的增加。
To develop a two-dimensional intravascular near-infrared fluorescence (NIRF) imaging strategy for investigation of arterial inflammation in coronary-sized vessels. Molecular imaging of arterial inflammation could provide new insights into the pathogenesis of acute myocardial infarction stemming from coronary atheromata and implanted stents. Presently few high-resolution inflammation approaches can image inflammation in coronary-sized arteries in vivo. A new 2.9F rotational, automated pullback two-dimensional imaging catheter was engineered and optimized for 360-degree viewing intravascular NIRF imaging. In conjunction with the cysteine protease-activatable imaging reporter Prosense VM110, intra-arterial 2D NIRF imaging was performed in rabbit aortas with atherosclerosis (n=10) or implanted coronary bare metal stents (n=10, 3.5mm diameter, day 7 post-implantation). Intravascular ultrasound (IVUS) provided co-registered anatomical images of arteries. After sacrifice, specimens underwent ex vivo NIRF imaging, fluorescence microscopy, and histological and immunohistochemical analyses. Imaging of coronary artery-scaled phantoms demonstrated 8-sector angular resolution and submillimeter axial resolution, nanomolar sensitivity to NIR fluorochromes, and modest NIRF light attenuation through blood. High-resolution NIRF images of vessel wall inflammation with signal-to-noise ratios > 10 were obtained in real-time through blood, without flushing or occlusion. In atherosclerosis, 2D NIRF, IVUS-NIRF fusion, microscopy, and immunoblotting studies provided insight into the spatial distribution of plaque protease activity. In stent-implanted vessels, real-time imaging illuminated an edge-based pattern of stent-induced arterial inflammation. A new 2D intravascular NIRF imaging strategy provides high-resolution in vivo spatial mapping of arterial inflammation in coronary-sized arteries, and reveals increased inflammation-regulated cysteine protease activity in atheromata and stentinduced arterial injury.
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影响因子：
3.6
作者：
Vinegoni C;Razansky D;Figueiredo JL;Nahrendorf M;Ntziachristos V;Weissleder R
通讯作者：
Weissleder R
蛋白水解活性动脉粥样硬化中炎症的实时导管分子传感。
影响因子：
37.8
作者：
Jaffer FA;Vinegoni C;John MC;Aikawa E;Gold HK;Finn AV;Ntziachristos V;Libby P;Weissleder R
通讯作者：
Weissleder R
影响因子：
37.8
作者：
Nakazawa, Gaku;Finn, Aloke V.;Virmani, Renu
通讯作者：
Virmani, Renu
影响因子：
64.8
作者：
Sanz, Javier;Fayad, Zahi A.
通讯作者：
Fayad, Zahi A.
影响因子：
3.8
作者：
Razansky, R. Nika;Rosenthal, Amir;Ntziachristos, Vasilis
通讯作者：
Ntziachristos, Vasilis