Porcine lactoferrin-derived peptide LFP-20 modulates immune homoeostasis to defend lipopolysaccharide-triggered intestinal inflammation in mice
Porcine lactoferrin-derived peptide LFP-20 modulates immune homoeostasis to defend lipopolysaccharide-triggered intestinal inflammation in mice
复制标题
猪乳铁蛋白衍生肽 LFP-20 调节免疫稳态以防御脂多糖引发的小鼠肠道炎症
DOI：
10.1017/s0007114519000485
复制
发表时间：
2019-03
影响因子：
3.6
通讯作者：
Yizhen Wang
中科院分区：
文献类型：
--
作者：
Xin Zong;Xiaoxuan Cao;Hong Wang;Jing Zhao;Zeqing Lu;Fengqin Wang;Yizhen Wang
Abstract The performance of immune system is vital for defending the body from pathogens, and it plays a crucial role in health homoeostasis. In a previous study, we have shown that LFP-20, a twenty-amino acid antimicrobial peptide in the N terminus of porcine lactoferrin, modulated inflammatory response in colitis. Here, we further investigated the effects of LFP-20 on immune homoeostasis to elucidate the mechanism of its anti-inflammation action. A lipopolysaccharide (LPS)-triggered systemic inflammatory response mice model was established. On the basis of observed mucosal lesions and apoptosis in small intestine, we found increased macrophage and neutrophil infiltration in ileum after LPS stimulation. Expectedly, LFP-20 pre-treatment attenuated the LPS-mediated immune disorders in ileum. Moreover, the flow cytometry results indicated pre-treatment with LFP-20 sustained the balance of CD3+CD8+ T cells, B cells and natural killer cells in LPS-triggered immune disturbance. Simultaneously, we demonstrated LFP-20 modulated the secretion of both activated Th1-related IL-12p70, interferon-γ, TNF-α and Th2-related IL-4, IL-5 and IL-6. Furthermore, we found LFP-20 facilitated a balanced Th1 and Th2 response, which triggered cellular defence mechanisms and induced B cells to produce opsonising antibodies belonging to certain IgG subclasses to defend against LPS stimulation. Collectively, our study indicated pre-treatment with LFP-20 could defend against LPS-triggered systemic inflammatory response in mice via modulating immune homoeostasis.
登录
查看更多内容
生理情况下单核细胞和组织驻留巨噬细胞的起源和稳态
影响因子：
5.6
作者：
Zhao Yang;Zou Weilong;Du Junfeng;Zhao Yong
通讯作者：
Zhao Yong
影响因子：
5.6
作者：
Rosa L;Cutone A;Lepanto MS;Paesano R;Valenti P
通讯作者：
Valenti P
影响因子：
2.1
作者：
Debbabi, H;Dubarry, M;Tomé, D
通讯作者：
Tomé, D
影响因子：
5.4
作者：
BELLAMY, W;WAKABAYASHI, H;TOMITA, M
通讯作者：
TOMITA, M
影响因子：
3.7
作者：
Shui-Bin Wang;Shui-Bin Wang;Yuqin Deng;Jie Ren;B. Xiao;Zhe Chen;Ze-Zhang Tao
通讯作者：
Shui-Bin Wang;Shui-Bin Wang;Yuqin Deng;Jie Ren;B. Xiao;Zhe Chen;Ze-Zhang Tao