DNA Sensing using Nano-crystalline Surface Enhanced Al(2)O(3) Nanopore Sensors.
DNA Sensing using Nano-crystalline Surface Enhanced Al(2)O(3) Nanopore Sensors.
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DOI：
10.1002/adfm.200902128
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发表时间：
2010-04-23
影响因子：
19
通讯作者：
Bashir, Rashid
中科院分区：
文献类型：
--
作者：
Venkatesan, Bala Murali;Shah, Amish B.;Zuo, Jian-Min;Bashir, Rashid
报道了一种新的固态Al 2 O3纳米孔传感器，具有增强的表面特性，用于实时检测和分析单个DNA分子。使用基于电子束的分解的纳米孔形成将孔的局部纳米结构和形态从无定形的化学计量结构（O与Al的比率为1.5）转变为异相结晶网络，缺乏O（O与Al的比率为~0.6）。在照射过程中观察到孔区域的直接金属化，从而允许在孔区域中潜在地制造纳米级金属接触，其具有基于纳米孔的DNA测序的潜在应用。在孔形成期间还观察到向纯γ和/或α相纳米微晶的剂量依赖性相变，从而允许在纳米孔/流体界面处进行表面电荷工程。DNA运输的研究表明，一个数量级的减少易位速度相对于备用固态架构，认可高表面电荷密度和成核的带电纳米晶域。Al 2 O3纳米孔传感器独特的表面特性使其成为检测和分析ssDNA、dsDNA、RNA二级结构和小蛋白质的理想选择。这些纳米级传感器也可以作为一个有用的工具，在研究驱动生物过程的机制，包括DNA-蛋白质相互作用和酶活性在单分子水平。
A new solid-state, Al2O3 nanopore sensor with enhanced surface properties for the real-time detection and analysis of individual DNA molecules is reported. Nanopore formation using electron beam based decomposition transformed the local nanostructure and morphology of the pore from an amorphous, stoichiometric structure (O to Al ratio of 1.5) to a hetero-phase crystalline network, deficient in O (O to Al ratio of ~0.6). Direct metallization of the pore region was observed during irradiation, thereby permitting the potential fabrication of nano-scale metallic contacts in the pore region with potential application to nanopore-based DNA sequencing. Dose dependent phase transformations to purely γ and/or α-phase nanocrystallites were also observed during pore formation allowing for surface charge engineering at the nanopore/fluid interface. DNA transport studies revealed an order of magnitude reduction in translocation velocities relative to alternate solid-state architectures, accredited to high surface charge density and the nucleation of charged nanocrystalline domains. The unique surface properties of Al2O3 nanopore sensors makes them ideal for the detection and analysis of ssDNA, dsDNA, RNA secondary structures and small proteins. These nano-scale sensors may also serve as a useful tool in studying the mechanisms driving biological processes including DNA-protein interactions and enzyme activity at the single molecule level.
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影响因子：
3.7
作者：
Iakoubovskii, Konstantin;Mitsuishi, Kazutaka;Furuya, Kazuo
通讯作者：
Furuya, Kazuo
影响因子：
3.4
作者：
Berezhkovskii, AM;Gopich, IV
通讯作者：
Gopich, IV
影响因子：
10.8
作者：
Fologea, D;Uplinger, J;Li, JL
通讯作者：
Li, JL
DOI：
10.1080/01418619808241915
发表时间：
1998-08-01
期刊：
PHILOSOPHICAL MAGAZINE A-PHYSICS OF CONDENSED MATTER STRUCTURE DEFECTS AND MECHANICAL PROPERTIES
影响因子：
--
作者：
Chen, GS;Boothroyd, CB;Humphreys, CJ
通讯作者：
Humphreys, CJ
影响因子：
10.8
作者：
Chang, H;Kosari, F;Bashir, R
通讯作者：
Bashir, R