Experimental identification and characterization of 97 novel npcRNA candidates in Salmonella enterica serovar Typhi.
Experimental identification and characterization of 97 novel npcRNA candidates in Salmonella enterica serovar Typhi.
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DOI：
10.1093/nar/gkq281
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发表时间：
2010-09
影响因子：
14.9
通讯作者：
Rozhdestvensky TS
中科院分区：
文献类型：
--
作者：
Chinni SV;Raabe CA;Zakaria R;Randau G;Hoe CH;Zemann A;Brosius J;Tang TH;Rozhdestvensky TS
我们从人类病原体伤寒沙门氏菌（以下简称伤寒沙门氏菌）中鉴定并鉴定了97种新的非蛋白编码RNA候选物（npcRNA）。伤寒）。3个是S.伤寒沙门氏菌中有22个，在伤寒沙门氏菌中有33个差异表达。伤寒生长。我们还鉴定了沙门氏菌致病岛来源的npcRNA，这些npcRNA可能参与沙门氏菌毒力、抗生素抗性和致病特异性的调节机制。伤寒。对S.伤寒StyR-3 npcRNA与沙门氏菌多药耐药（MDR）相关基因拉马的转录抑制因子RamR相互作用。StyR-3干扰RamR-DNA结合活性，因此可能在调节拉马基因表达中起作用，导致MDR表型。我们的研究还揭示了大量的顺式编码的反义npcRNA候选者，支持以前在细菌中观察到的全局正义-反义调控网络。最后，至少有六个npcRNA候选物与S. Typhi Hfq蛋白，支持Hfq在npcRNA网络中的重要作用。这项研究指出了新的功能npcRNA候选人可能参与各种调控作用，包括致病性的S。伤寒。
We experimentally identified and characterized 97 novel, non-protein-coding RNA candidates (npcRNAs) from the human pathogen Salmonella enterica serovar Typhi (hereafter referred to as S. typhi). Three were specific to S. typhi, 22 were restricted to Salmonella species and 33 were differentially expressed during S. typhi growth. We also identified Salmonella Pathogenicity Island-derived npcRNAs that might be involved in regulatory mechanisms of virulence, antibiotic resistance and pathogenic specificity of S. typhi. An in-depth characterization of S. typhi StyR-3 npcRNA showed that it specifically interacts with RamR, the transcriptional repressor of the ramA gene, which is involved in the multidrug resistance (MDR) of Salmonella. StyR-3 interfered with RamR–DNA binding activity and thus potentially plays a role in regulating ramA gene expression, resulting in the MDR phenotype. Our study also revealed a large number of cis-encoded antisense npcRNA candidates, supporting previous observations of global sense–antisense regulatory networks in bacteria. Finally, at least six of the npcRNA candidates interacted with the S. typhi Hfq protein, supporting an important role of Hfq in npcRNA networks. This study points to novel functional npcRNA candidates potentially involved in various regulatory roles including the pathogenicity of S. typhi.
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影响因子：
2.9
作者：
Iben, James R.;Draper, David E.
通讯作者：
Draper, David E.
DOI：
10.1073/pnas.0600927103
发表时间：
2006-05-02
影响因子：
11.1
作者：
Dühring, U;Axmann, IM;Wilde, A
通讯作者：
Wilde, A
影响因子：
56.9
作者：
Kapranov, Philipp;Cheng, Jill;Gingeras, Thomas R.
通讯作者：
Gingeras, Thomas R.
影响因子：
64.8
作者：
CRAIGEN, WJ;CASKEY, CT
通讯作者：
CASKEY, CT
影响因子：
4.4
作者：
Gonzalez, Nicolas;Heeb, Stephan;Valverde, Claudio;Kay, Elisabeth;Reimmann, Cornelia;Junier, Thomas;Haas, Dieter
通讯作者：
Haas, Dieter