Genome-wide search reveals a novel GacA-regulated small RNA in Pseudomonas species.
Genome-wide search reveals a novel GacA-regulated small RNA in Pseudomonas species.
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DOI：
10.1186/1471-2164-9-167
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发表时间：
2008-04-13
期刊：
影响因子：
4.4
通讯作者：
Haas, Dieter
中科院分区：
文献类型：
--
作者：
Gonzalez, Nicolas;Heeb, Stephan;Valverde, Claudio;Kay, Elisabeth;Reimmann, Cornelia;Junier, Thomas;Haas, Dieter
小 RNA (sRNA) 在细菌中广泛存在，并具有多种调节作用。大多数 sRNA 是通过计算和实验方法相结合发现的。在铜绿假单胞菌（一种普遍存在的革兰氏阴性细菌和机会性人类病原体）中，GacS/GacA 双组分系统正向控制两种 sRNA（RsmY、RsmZ）的转录，这对于毒力相关基因的表达至关重要。在生物防治细菌荧光假单胞菌 CHA0 中，三种 GacA 控制的 sRNA（RsmX、RsmY、RsmZ）调节对氧化应激的反应以及包括生物防治因子在内的细胞外产物的表达。 RsmX、RsmY 和 RsmZ 包含多个未配对的 GGA 基序，并通过隔离 RsmA 家族的翻译抑制蛋白在翻译水平控制靶 mRNA 的表达。计算和实验相结合的方法使我们能够识别铜绿假单胞菌中编码 sRNA 的 14 个基因间区域。其中 8 个区域编码新鉴定的 sRNA。基因间区域 1698 被发现指定了一种新的 GacA 控制的 sRNA，称为 RgsA。 GacA 的调节似乎是间接的。在荧光假单胞菌 CHA0 中，RgsA 同源物也在阳性 GacA 对照下表达。这种 120 nt 的 sRNA 含有单个 GGA 基序，与 RsmX、RsmY 和 RsmZ 不同，它无法去抑制 hcnA 基因（参与生物防治因子氰化氢的生物合成）的翻译，但有助于细菌对过氧化氢的抗性。在铜绿假单胞菌和荧光假单胞菌中，应激西格玛因子 RpoS 对于 RgsA 表达至关重要。在两种假单胞菌属物种中发现在 GacA 控制下表达的额外 sRNA 突显了这一全球调控系统的复杂性，并表明 GacA 控制的作用模式可能比之前怀疑的更为复杂。我们的结果还证实，sRNA 中需要多个 GGA 基序来隔离 RsmA 蛋白。
Small RNAs (sRNAs) are widespread among bacteria and have diverse regulatory roles. Most of these sRNAs have been discovered by a combination of computational and experimental methods. In Pseudomonas aeruginosa, a ubiquitous Gram-negative bacterium and opportunistic human pathogen, the GacS/GacA two-component system positively controls the transcription of two sRNAs (RsmY, RsmZ), which are crucial for the expression of genes involved in virulence. In the biocontrol bacterium Pseudomonas fluorescens CHA0, three GacA-controlled sRNAs (RsmX, RsmY, RsmZ) regulate the response to oxidative stress and the expression of extracellular products including biocontrol factors. RsmX, RsmY and RsmZ contain multiple unpaired GGA motifs and control the expression of target mRNAs at the translational level, by sequestration of translational repressor proteins of the RsmA family. A combined computational and experimental approach enabled us to identify 14 intergenic regions encoding sRNAs in P. aeruginosa. Eight of these regions encode newly identified sRNAs. The intergenic region 1698 was found to specify a novel GacA-controlled sRNA termed RgsA. GacA regulation appeared to be indirect. In P. fluorescens CHA0, an RgsA homolog was also expressed under positive GacA control. This 120-nt sRNA contained a single GGA motif and, unlike RsmX, RsmY and RsmZ, was unable to derepress translation of the hcnA gene (involved in the biosynthesis of the biocontrol factor hydrogen cyanide), but contributed to the bacterium's resistance to hydrogen peroxide. In both P. aeruginosa and P. fluorescens the stress sigma factor RpoS was essential for RgsA expression. The discovery of an additional sRNA expressed under GacA control in two Pseudomonas species highlights the complexity of this global regulatory system and suggests that the mode of action of GacA control may be more elaborate than previously suspected. Our results also confirm that several GGA motifs are required in an sRNA for sequestration of the RsmA protein.
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影响因子：
14.9
作者：
Afonyushkin, T;Vecerek, B;Moll, I;Bläsi, U;Kaberdin, VR
通讯作者：
Kaberdin, VR
影响因子：
14.9
作者：
Ding, Y;Chan, CY;Lawrence, CE
通讯作者：
Lawrence, CE
影响因子：
2.1
作者：
Heeb, S;Valverde, C;Haas, D
通讯作者：
Haas, D
DOI：
10.1073/pnas.1731982100
发表时间：
2003-09-02
影响因子：
11.1
作者：
Buell, CR;Joardar, V;Collmer, A
通讯作者：
Collmer, A
影响因子：
3.2
作者：
Heeb, S;Blumer, C;Haas, D
通讯作者：
Haas, D