Identification of Molecular Mechanism of OSA with Hypertension Based on Multiple Microarray Analysis
Identification of Molecular Mechanism of OSA with Hypertension Based on Multiple Microarray Analysis
复制标题
基于多重微阵列分析识别 OSA 合并高血压的分子机制
DOI：
10.1155/2022/1257400
复制
发表时间：
2022-07
影响因子：
--
通讯作者：
Xiaoling Gao
中科院分区：
文献类型：
--
作者：
Xizhu Zhang;Gang Jing;Liqing Qi;Mingzhu Ma;Liting Li;Ningning Shen;Zheng Guo;Xiaoling Gao
目的.阻塞性睡眠呼吸暂停与高血压密切相关，流行病学和实验研究均证实阻塞性睡眠呼吸暂停是高血压最重要的独立危险因素之一。OSA引起高血压的病理机制目前还不清楚，本文从生物信息学水平探讨了OSA介导高血压的分子机制。材料与方法。我们从GEO公共数据库下载疾病相关数据集，通过limma包计算两组患者的差异基因，然后进一步构建基于患者临床特征的WGCNA网络，探索疾病中的重要调控基因。随后，ssGSEA被用于探索疾病进展的潜在分子机制，GSVA被用于分析特定的信号通路。最后，我们进行了实时定量PCR（qRT-PCR），以验证这些关键基因。结果选择三个基因作为靶基因，即GPR 179、RNF 150和JPH 4。结果表明，它们与免疫细胞含量密切相关，这三个核心基因的高表达与肌生成、血管生成、氧化、代谢和PI 3 K/AKT/mTOR通路有关。qRT-PCR验证了3个基因在OSA组和OSA合并高血压组之间的差异有统计学意义。结论本研究为OSA合并高血压病的分子机制及诊断和治疗提供了新的证据。
Purpose. OSA is closely associated with hypertension, and both epidemiological and experimental studies have confirmed that OSA is one of the most important independent risk factors for hypertension. The pathological mechanisms by which OSA causes hypertension are not well understood, and in this paper, we explored the molecular mechanisms by which OSA may mediate hypertension at the bioinformatics level. Materials and Methods. We downloaded disease-related datasets from the GEO public database, calculated the differential genes between the two groups of patients by the limma package, and then further constructed the WGCNA network based on the clinical characteristics of patients to explore the important regulatory genes in the disease. Subsequently, ssGSEA was used to explore the potential molecular mechanisms of disease progression and GSVA was applied to analyze the specific signaling pathways. Finally, we performed real-time quantitative PCR (qRT-PCR) to validate these pivotal genes. Results. Three genes were selected as target genes, namely, GPR179, RNF150, and JPH4. The results showed they were strongly correlated with immune cell content that high expression of the three core genes was associated with myogenesis, angiogenesis, oxidation, metabolism, and PI3K/AKT/mTOR pathways. qRT-PCR validated that all three genes have statistically significant differences between the OSA group and OSA combined with hypertension group. Conclusion. Our study provides new evidence for the potential molecular mechanisms of OSA combined with hypertensive disease as well as diagnosis and treatment.
登录
查看更多内容
EGLN2 和 RNF150 基因变异与中国人群慢性阻塞性肺疾病风险相关。
DOI：
10.2147/copd.s73031
发表时间：
2015
影响因子：
2.8
作者：
Ding Y;Niu H;Yang H;Sun P;Chen Y;Duan M;Xu D;Xu J;Jin T
通讯作者：
Jin T
影响因子：
14.9
作者：
Ritchie ME;Phipson B;Wu D;Hu Y;Law CW;Shi W;Smyth GK
通讯作者：
Smyth GK
从哺乳动物基因表达数据中发现组织特异性DNA序列基序的荟萃分析发现。
影响因子：
3
作者：
Huber, Bertrand R.;Bulyk, Martha L.
通讯作者：
Bulyk, Martha L.
影响因子：
4.7
作者：
Ohayon, MM;Guilleminault, C;Smirne, S
通讯作者：
Smirne, S
影响因子：
56.9
作者：
Ravasz, E;Somera, AL;Barabási, AL
通讯作者：
Barabási, AL