The cytoprotective role of DJ-1 and p45 NFE2 against human primary alveolar type II cell injury and emphysema.
The cytoprotective role of DJ-1 and p45 NFE2 against human primary alveolar type II cell injury and emphysema.
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DOI：
10.1038/s41598-018-21790-3
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发表时间：
2018-02-23
影响因子：
4.6
通讯作者：
Kosmider B
中科院分区：
文献类型：
--
作者：
Tan LH;Bahmed K;Lin CR;Marchetti N;Bolla S;Criner GJ;Kelsen S;Madesh M;Kosmider B
Emphysema is characterized by irreversibly enlarged airspaces and destruction of alveolar walls. One of the factors contributing to this disease pathogenesis is an elevation in extracellular matrix (ECM) degradation in the lung. Alveolar type II (ATII) cells produce and secrete pulmonary surfactants and proliferate to restore the epithelium after damage. We isolated ATII cells from control non-smokers, smokers and patients with emphysema to determine the role of NFE2 (nuclear factor, erythroid-derived 2). NFE2 is a heterodimer composed of two subunits, a 45 kDa (p45 NFE2) and 18 kDa (p18 NFE2) polypeptides. Low expression of p45 NFE2 in patients with emphysema correlated with a high ECM degradation. Moreover, we found that NFE2 knockdown increased cell death induced by cigarette smoke extract. We also studied the cross talk between p45 NFE2 and DJ-1. DJ-1 protein is a redox-sensitive chaperone that protects cells from oxidative stress. We detected that cigarette smoke significantly increased p45 NFE2 levels in DJ-1 KO mice compared to wild-type mice. Our results indicate that p45 NFE2 expression is induced by exposure to cigarette smoke, has a cytoprotective activity against cell injury, and its downregulation in human primary ATII cells may contribute to emphysema pathogenesis.
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影响因子：
5.8
作者：
Gondi CS;Rao JS
通讯作者：
Rao JS
影响因子：
6.9
作者：
Houghton, A. McGarry
通讯作者：
Houghton, A. McGarry
DOI：
10.1164/rccm.200506-953oc
发表时间：
2005-12-01
影响因子：
24.7
作者：
Ito, I;Nagai, S;Mishima, M
通讯作者：
Mishima, M
影响因子：
5.8
作者：
Kosmider B;Messier EM;Janssen WJ;Nahreini P;Wang J;Hartshorn KL;Mason RJ
通讯作者：
Mason RJ
DOI：
10.1164/ajrccm.159.6.9809043
发表时间：
1999-06-01
影响因子：
24.7
作者：
Betsuyaku, T;Nishimura, M;Kawakami, Y
通讯作者：
Kawakami, Y