Erectile function is improved in aged rats by PnTx2-6, a toxin from Phoneutria nigriventer spider venom.
Erectile function is improved in aged rats by PnTx2-6, a toxin from Phoneutria nigriventer spider venom.
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DOI：
10.1111/j.1743-6109.2012.02878.x
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发表时间：
2012-10
期刊：
影响因子：
--
通讯作者：
de Lima ME
中科院分区：
文献类型：
--
作者：
Nunes KP;Toque HA;Borges MH;Richardson M;Webb RC;de Lima ME
介绍性勃起功能障碍（艾德）涉及一氧化氮（NO）可用性降低和松弛受损。PnTx 2 -6是一种来自黑腹拟黑胸蛛的毒素，已被证明通过NO/环磷酸鸟苷（cGMP）途径改善勃起功能。这种蜘蛛的毒液有几种症状，包括勃起。PnTx 2 -6与这种现象有关。动物毒液被认为是治疗ED的潜在药物。AimPnTx 2 -6毒素通过NO/cGMP改善老年大鼠的勃起功能。我们研究了PnTx 2 -6在老年大鼠勃起功能中的作用。主要结果通过电场刺激（EFS）老年和成年大鼠（70 vs. 14周）期间海绵体内压/平均动脉压比值的变化来评估MeasuresED。在功能研究中，EFS-induced松弛的阴茎海绵体（CC）条进行或不PnTx 2 -6（10- 8 M）。ResultsThe下降与年龄相关的勃起功能部分恢复15-20分钟后，注射PnTx 2 -6和进一步改善西地那非。PnTx 2 -6增强EFS-induced松弛，以及在CC cGMP水平，从年轻和老年大鼠。在老化CC中，与Rho激酶抑制剂Y-27632（10-6 M）孵育30分钟后，由于PnTx 2 -6引起的松弛进一步增加。一氧化氮合酶（NOS）的活性在老年人和年轻的海绵体组织与PnTx 2 -6孵育（10分钟）增加。结论PnTx 2 -6通过增加NOS活性和NO释放，提高cGMP水平，改善老年大鼠阴茎的舒张功能。
IntroductionAge-associated erectile dysfunction (ED) involves a decrease in nitric oxide (NO) availability and impaired relaxation. PnTx2-6, a toxin from thePhoneutria nigriventerspider, has been demonstrated to improve erectile function via NO/cyclic guanosine monophosphate (cGMP) pathway. This spider’s venom is characterized by several symptoms, including erection. PnTx2-6 has been implicated in this phenomenon. Animal venoms have been postulated as potential drugs to treat ED.AimPnTx2-6 toxin improves erectile function in aged rats via NO/cGMP. We investigated the effect of PnTx2-6 in the erectile function of aged rats.Main Outcome MeasuresED was evaluated through changes in intracavernosal pressure/mean arterial pressure ratio during electrical field stimulation (EFS) of the pelvic ganglion of aged and adult rats (70 vs. 14 weeks). In functional studies, EFS-induced relaxation of corpus cavernosum (CC) strips were performed with or without PnTx2-6 (10-8M).ResultsThe decrease in erectile function associated with age was partially restored 15–20 minutes after injection of PnTx2-6 and further improved by sildenafil. PnTx2-6 enhanced EFS-induced relaxation, as well as cGMP levels in CC, from young and aged rats. Relaxation due to PnTx2-6 was further increased after 30 minutes incubation with Y-27632, a Rho-kinase inhibitor (10-6 M), in aging CC. Nitric oxide synthase (NOS) activity in aged and young cavernosal tissue was increased by incubation with PnTx2-6 (10 minutes). However, this toxin did not modify NOS expression.ConclusionPnTx2-6 improves penile relaxation in aged rats, via increased NOS activity and NO release, resulting in enhanced cGMP levels.
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影响因子：
4.5
作者：
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通讯作者：
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