Brown planthopper (N. lugens Stal) feeding behaviour on rice germplasm as an indicator of resistance.
Brown planthopper (N. lugens Stal) feeding behaviour on rice germplasm as an indicator of resistance.
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DOI：
10.1371/journal.pone.0022137
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发表时间：
2011
期刊：
影响因子：
3.7
通讯作者：
Ford-Lloyd B
中科院分区：
文献类型：
--
作者：
Ghaffar MB;Pritchard J;Ford-Lloyd B
褐飞虱Nilaparvata lugens（Stal）是亚洲水稻的重要害虫。新的控制策略的发展，可以促进通过比较BPH的喂养行为表现出自然的遗传变异的品种，然后阐明的基本机制的电阻。利用电穿透图（EPG）和蜜露钟比较了褐飞虱在12个水稻品种上12 h的取食行为。七个摄食行为（波形）被确定，并可分为两个阶段。第一个阶段涉及筛元定位模式，包括非穿透（NP）、通道（N1+N2+N3）、木质部（N5）和两种新的摄食波形，即偏离口针力学（N6）和细胞穿透（N7）。第二个摄食阶段由分泌唾液进入筛元件（N4-a）和筛元件汁液摄取（N4-B）组成。蜜露滴的生产与N4-B波形模式提供独立的确认这种喂养行为。取食行为的总体变化与先前发表的不同水稻品种的田间抗性或敏感性高度相关：褐飞虱产生的蜜露滴数较低，抗性水稻品种的韧皮部取食期较短，但第一次流涎的时间（N4-B）没有显著差异。这些定性的行为差异表明，阻力是由持续的韧皮部摄入的差异，而不是韧皮部的位置。对取食和蜜露数据的聚类分析将12个水稻品种分为三组：敏感、中抗和高抗。我们所描述的筛选方法揭示了水稻对BPH的抗性机制（或机制）的新方面，并将与分子方法相结合，以识别和开发新的控制策略。
The brown planthopper (BPH) Nilaparvata lugens (Stal) is a serious pest of rice in Asia. Development of novel control strategies can be facilitated by comparison of BPH feeding behaviour on varieties exhibiting natural genetic variation, and then elucidation of the underlying mechanisms of resistance. BPH feeding behaviour was compared on 12 rice varieties over a 12 h period using the electrical penetration graph (EPG) and honeydew clocks. Seven feeding behaviours (waveforms) were identified and could be classified into two phases. The first phase involved patterns of sieve element location including non penetration (NP), pathway (N1+N2+N3), xylem (N5) and two new feeding waveforms, derailed stylet mechanics (N6) and cell penetration (N7). The second feeding phase consisted of salivation into the sieve element (N4-a) and sieve element sap ingestion (N4-b). Production of honeydew drops correlated with N4-b waveform patterns providing independent confirmation of this feeding behaviour. Overall variation in feeding behaviour was highly correlated with previously published field resistance or susceptibility of the different rice varieties: BPH produced lower numbers of honeydew drops and had a shorter period of phloem feeding on resistant rice varieties, but there was no significant difference in the time to the first salivation (N4-b). These qualitative differences in behaviour suggest that resistance is caused by differences in sustained phloem ingestion, not by phloem location. Cluster analysis of the feeding and honeydew data split the 12 rice varieties into three groups: susceptible, moderately resistant and highly resistant. The screening methods that we have described uncover novel aspects of the resistance mechanism (or mechanisms) of rice to BPH and will in combination with molecular approaches allow identification and development of new control strategies.
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