Sex pheromone receptor specificity in the European corn borer moth, Ostrinia nubilalis.
Sex pheromone receptor specificity in the European corn borer moth, Ostrinia nubilalis.
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欧洲玉米虫蛾的性信息素受体特异性，奥斯坦尼亚nubilalis。
DOI：
10.1371/journal.pone.0008685
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发表时间：
2010-01-13
期刊：
影响因子：
3.7
通讯作者：
Luetje CW
中科院分区：
文献类型：
--
作者：
Wanner KW;Nichols AS;Allen JE;Bunger PL;Garczynski SF;Linn CE;Robertson HM;Luetje CW
欧洲玉米螟Ostrinia nubilaris(Hubner)以两种不同的性信息素小种存在。ECB(Z)雌虫产生Z11-和E11-十四烯基乙酸酯的混合物为97：3，而ECB(E)雌虫产生Z和E异构体的比例为1：99。每个种族的雄性对其同种雌性的混合都有特殊的反应。作为一种近缘物种，亚洲玉米螟(ACB)使用Z12-和E12-十四烯基乙酸酯的3：2混合物，被认为是从类似欧洲央行的祖先进化而来的。为了进一步了解信息素检测的分子机制及其在亲缘关系密切的物种中的进化，我们鉴定并鉴定了ECB(Z)的性信息素受体。采用同源依赖(设计简并的氨基酸基序的简并聚合酶链式反应)和同源非依赖的方法(触角的焦磷酸测序)来鉴定候选的性信息素转录本。用实时荧光定量聚合酶链式反应检测雌雄触角的表达。采用双电极电压钳电生理学方法对非洲爪哇卵母细胞表达的候选受体进行了功能研究。我们鉴定了五种性信息素受体OnOrs1和OnOrs3-6。它们在雄性触角中的转录丰度是雌性触角的14-100倍。OnOr6对Z11-十四烯基乙酸酯有很高的选择性(EC_(50) = 为0.86±0.27微米)，而对E11-十四烯基乙酸酯的反应至少低三个数量级。令人惊讶的是，OnOr1、OnOr3和OnOr5对所有测试的四种信息素(Z11-和E11-十四烯基醋酸酯，以及Z12-和E12-十四烯基醋酸酯)以及行为拮抗剂Z9-十四烯基醋酸酯都有反应。OnOr1对E12-十四烯基乙酸酯具有选择性，其疗效至少是其他四种成分的5倍。这种特异性和广泛性信息素受体的组合与已发表的感觉神经元活体活动结果相一致。受体对一类信息素成分的广泛反应可能为雄蛾反应的变异提供了一种机制，使信息素混合使用中的种群水平发生变化。
The European corn borer (ECB), Ostrinia nubilalis (Hubner), exists as two separate sex pheromone races. ECB(Z) females produce a 97∶3 blend of Z11- and E11-tetradecenyl acetate whereas ECB(E) females produce an opposite 1∶99 ratio of the Z and E isomers. Males of each race respond specifically to their conspecific female's blend. A closely related species, the Asian corn borer (ACB), O. furnacalis, uses a 3∶2 blend of Z12- and E12-tetradecenyl acetate, and is believed to have evolved from an ECB-like ancestor. To further knowledge of the molecular mechanisms of pheromone detection and its evolution among closely related species we identified and characterized sex pheromone receptors from ECB(Z). Homology-dependent (degenerate PCR primers designed to conserved amino acid motifs) and homology-independent (pyrophosphate sequencing of antennal cDNA) approaches were used to identify candidate sex pheromone transcripts. Expression in male and female antennae was assayed by quantitative real-time PCR. Two-electrode voltage clamp electrophysiology was used to functionally characterize candidate receptors expressed in Xenopus oocytes. We characterized five sex pheromone receptors, OnOrs1 and 3–6. Their transcripts were 14–100 times more abundant in male compared to female antennae. OnOr6 was highly selective for Z11-tetradecenyl acetate (EC50 = 0.86±0.27 µM) and was at least three orders of magnitude less responsive to E11-tetradecenyl acetate. Surprisingly, OnOr1, 3 and 5 responded to all four pheromones tested (Z11- and E11-tetradecenyl acetate, and Z12- and E12-tetradecenyl acetate) and to Z9-tetradecenyl acetate, a behavioral antagonist. OnOr1 was selective for E12-tetradecenyl acetate based on an efficacy that was at least 5-fold greater compared to the other four components. This combination of specifically- and broadly-responsive pheromone receptors corresponds to published results of sensory neuron activity in vivo. Receptors broadly-responsive to a class of pheromone components may provide a mechanism for variation in the male moth response that enables population level shifts in pheromone blend use.
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影响因子：
9.8
作者：
Benton, Richard;Sachse, Silke;Michnick, Stephen W;Vosshall, Leslie B
通讯作者：
Vosshall, Leslie B
影响因子：
2.2
作者：
HANSSON, BS;BLACKWELL, A;LOFQVIST, J
通讯作者：
LOFQVIST, J
DOI：
10.1017/cbo9780511542664.009
发表时间：
2004-01-01
期刊：
ADVANCES IN INSECT CHEMICAL ECOLOGY
影响因子：
--
作者：
Cardé, RT;Haynes, KF
通讯作者：
Haynes, KF
影响因子：
2.8
作者：
Karpati, Zsolt;Dekker, Teun;Hansson, Bill S.
通讯作者：
Hansson, Bill S.
影响因子：
1.9
作者：
Ishikawa, Y;Takanashi, T;Huang, YP
通讯作者：
Huang, YP