Genetic fate-mapping reveals surface accumulation but not deep organ invasion of pleural and peritoneal cavity macrophages following injury.
Genetic fate-mapping reveals surface accumulation but not deep organ invasion of pleural and peritoneal cavity macrophages following injury.
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基因命运图谱揭示了损伤后胸膜和腹膜腔巨噬细胞的表面积累，但没有深入器官侵袭
DOI：
10.1038/s41467-021-23197-7
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发表时间：
2021-05-17
影响因子：
16.6
通讯作者：
Zhou B
中科院分区：
文献类型：
--
作者：
Jin H;Liu K;Tang J;Huang X;Wang H;Zhang Q;Zhu H;Li Y;Pu W;Zhao H;He L;Li Y;Zhang S;Zhang Z;Zhao Y;Qin Y;Pflanz S;Kasmi KEI;Zhang W;Liu Z;Ginhoux F;Ji Y;He B;Wang L;Zhou B
在损伤期间，单核细胞从循环募集到发炎组织并局部分化成成熟巨噬细胞，先前的报道显示腹膜和心包的腔巨噬细胞深入侵入各自的器官以促进修复。在这里，我们报告了一个双重重组酶介导的遗传系统，旨在跟踪腔巨噬细胞在体内的交叉检测两个特征标记。用这种方法进行的谱系追踪显示，在肺和肝损伤期间，空腔巨噬细胞在内脏器官表面积聚，而没有渗透到实质中。其他数据表明，这些腹膜或胸膜腔巨噬细胞不有助于组织修复和再生。因此，我们的体内遗传靶向方法提供了一种可靠的方法来鉴定和表征腔巨噬细胞在其发育和组织修复和再生过程中的特征，并将这些细胞与其他谱系区分开来。
During injury, monocytes are recruited from the circulation to inflamed tissues and differentiate locally into mature macrophages, with prior reports showing that cavity macrophages of the peritoneum and pericardium invade deeply into the respective organs to promote repair. Here we report a dual recombinase-mediated genetic system designed to trace cavity macrophages in vivo by intersectional detection of two characteristic markers. Lineage tracing with this method shows accumulation of cavity macrophages during lung and liver injury on the surface of visceral organs without penetration into the parenchyma. Additional data suggest that these peritoneal or pleural cavity macrophages do not contribute to tissue repair and regeneration. Our in vivo genetic targeting approach thus provides a reliable method to identify and characterize cavity macrophages during their development and in tissue repair and regeneration, and distinguishes these cells from other lineages.
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影响因子：
15.3
作者：
VANFURTH, R;DIESSELH.MM
通讯作者：
DIESSELH.MM
DOI：
10.1084/jem.20141539
发表时间：
2015-04-06
期刊：
The Journal of experimental medicine
影响因子：
--
作者：
Dal-Secco D;Wang J;Zeng Z;Kolaczkowska E;Wong CH;Petri B;Ransohoff RM;Charo IF;Jenne CN;Kubes P
通讯作者：
Kubes P
影响因子：
30.5
作者：
通讯作者：
--
影响因子：
64.8
作者：
Dor, Y;Brown, J;Melton, DA
通讯作者：
Melton, DA
影响因子：
48
作者：
Buch, T;Heppner, FL;Waisman, A
通讯作者：
Waisman, A