The kinetics of promonocytes and monocytes in the bone marrow.
The kinetics of promonocytes and monocytes in the bone marrow.
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DOI：
10.1084/jem.132.4.813
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发表时间：
1970-10-01
影响因子：
15.3
通讯作者：
DIESSELH.MM
中科院分区：
文献类型：
--
作者：
VANFURTH, R;DIESSELH.MM
骨髓的单核吞噬细胞可分为两种细胞类型：单核细胞和单核细胞。本研究旨在确定单核细胞是否是单核细胞的祖细胞，并确定骨髓腔室中单核细胞和单核细胞的动力学特征。胸腺嘧啶- 3h体外标记研究和单个细胞细胞核中DNA相对量的测定表明，在正常稳态条件下，单核细胞为增殖细胞，单核细胞为非分裂细胞。体内标记研究进一步证明了前单核细胞是单核细胞的祖细胞。在标记后的前24小时内，原细胞显示出持续高水平的标记（约70%）。这些细胞的平均粒数随着时间的延长而减少。在体内标记后的前24小时，骨髓单核细胞的标记指数增加，但在同一时期，平均粒数几乎保持不变，实验前6小时的数值约为原细胞的一半。利用骨髓中原单核细胞和单核细胞的标记指数和百分比分布比数据，以及外周血单核细胞的标记指数构建模型群体。结果得出结论，促母细胞是增殖细胞，由一个促母细胞分裂产生的两个子细胞都是单核细胞。原细胞的dna合成时间为13.6小时。根据该值，计算出平均生成时间为19.5小时。
The mononuclear phagocytes of the bone marrow can be classified into two cell types, promonocytes and monocytes. The present study was performed to establish whether the promonocytes are the progenitors of the monocytes and to determine the kinetic characteristics of the promonocytes and monocytes in the bone marrow compartment. Both in vitro labeling studies with thymidine-3H and determination of the relative amount of DNA in the nuclei of individual cells showed that under normal steady-state conditions the promonocytes are proliferating cells and the monocytes, nondividing cells. In vivo labeling studies provided further evidence that the promonocytes are the progenitor cells of the monocytes. During the first 24 hr after labeling, the promonocytes showed a constant high level of labeling (about 70%). The mean grain count of these cells decreased with time. The labeling index of the monocytes of the bone marrow increased during the first 24 hr after in vivo labeling, but during the same period the mean grain counts remained almost constant, with values amounting to about half those of the promonocytes during the first 6 hr of the experiment. The data concerning the labeling indices and the percentage distribution ratio of the promonocytes and monocytes in the bone marrow, and the labeling indices of the peripheral blood monocytes are used to construct a model population. The results lead to the conclusions that the promonocytes are multiplicative cells and that both daughter cells arising from the division of a promonocyte are monocytes. The DNA-synthesis time found for the promonocytes is 13.6 hr. From this value, the average generation time was computed to be 19.5 hr.
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单核吞噬细胞的起源和动力学。
影响因子：
15.3
作者：
van Furth, R;Cohn, Z A
通讯作者：
Cohn, Z A
影响因子：
3.2
作者：
DECOSSE, JJ;AIELLO, N
通讯作者：
AIELLO, N
DOI：
10.3181/00379727-75-18083
发表时间：
1950-01-01
影响因子：
--
作者：
KALISS, N;PRESSMAN, D
通讯作者：
PRESSMAN, D
反应性裂解：补体介导的未敏化细胞的裂解。 I.指标因子的表征及其标识为C7。
影响因子：
15.3
作者：
THOMPSON, RA;LACHMANN, PJ
通讯作者：
LACHMANN, PJ
影响因子：
15.3
作者：
VANFURTH, R;HIRSCH, JG;FEDORKO, ME
通讯作者：
FEDORKO, ME