Simple sequence repeats in Haemophilus influenzae.
Simple sequence repeats in Haemophilus influenzae.
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DOI：
10.1016/j.meegid.2008.11.006
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发表时间：
2009-03
期刊：
影响因子：
--
通讯作者：
Hood DW
中科院分区：
文献类型：
--
作者：
Power PM;Sweetman WA;Gallacher NJ;Woodhall MR;Kumar GA;Moxon ER;Hood DW
DNA的简单重复序列（SSR）易发生高频率突变，是流感嗜血杆菌适应的重要介质。先前对Rd KW 20基因组的研究确定了四核苷酸SSR在介导表面暴露结构（如脂多糖）的相位变化（基因表达的快速可逆转换）中的首要地位。最近对多株H.流感病毒允许在四个完整的H中详细比较SSR（长度为1至9个核苷酸的重复单元）。流感病毒基因组，然后与另外12个基因组进行比较，当他们变得可用。SSR位点大致分为三组：（1）没有变化的;（2）在菌株之间观察到一些变化，但这不能与基因表达的变化联系起来的;和（3）既变化又位于与介导相变量基因表达一致的区域的。通过对988个SSR相关位点的比较分析，证实了四核苷酸重复序列是相位变异的主要介导者，并通过鉴定10个先前未鉴定的具有介导相位变异潜力的四核苷酸SSR位点，扩展了已知四核苷酸SSR位点的库，这些位点在整个H.流感全基因组此外，在16个菌株中的非四核苷酸SSR分析揭示了一些单核苷酸，二核苷酸，五核苷酸，七核苷酸，和八核苷酸SSR与这些道介导的相位变化是一致的。这项研究证实了以前的研究结果，四核苷酸SSR在H。流感生物学两个巴西菌株表现出最大的变化，在其补充SSR的地理和表型的影响SSR分布的可能性。
Simple sequence repeat (SSRs) of DNA are subject to high rates of mutation and are important mediators of adaptation in Haemophilus influenzae. Previous studies of the Rd KW20 genome identified the primacy of tetranucleotide SSRs in mediating phase variation (the rapid reversible switching of gene expression) of surface exposed structures such as lipopolysaccharide. The recent sequencing of the genomes of multiple strains of H. influenzae allowed the comparison of the SSRs (repeat units of one to nine nucleotides in length) in detail across four complete H. influenzae genomes and then comparison with a further 12 genomes when they became available. The SSR loci were broadly classified into three groups: (1) those that did not vary; (2) those for which some variation between strains was observed but this could not be linked to variation of gene expression; and (3) those that both varied and were located in regions consistent with mediating phase variable gene expression. Comparative analysis of 988 SSR associated loci confirmed that tetranucleotide repeats were the major mediators of phase variation and extended the repertoire of known tetranucleotide SSR loci by identifying ten previously uncharacterised tetranucleotide SSR loci with the potential to mediate phase variation which were unequally distributed across the H. influenzae pan-genome. Further, analysis of non-tetranucleotide SSR in the 16 strains revealed a number of mononucleotide, dinucleotide, pentanucleotide, heptanucleotide, and octanucleotide SSRs which were consistent with these tracts mediating phase variation. This study substantiates previous findings as to the important role that tetranucleotide SSRs play in H. influenzae biology. Two Brazilian isolates showed the most variation in their complement of SSRs suggesting the possibility of geographic and phenotypic influences on SSR distribution.
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影响因子：
12.3
作者：
Hogg JS;Hu FZ;Janto B;Boissy R;Hayes J;Keefe R;Post JC;Ehrlich GD
通讯作者：
Ehrlich GD
影响因子：
3.1
作者：
BARENKAMP, SJ;LEININGER, E
通讯作者：
LEININGER, E
DOI：
10.1073/pnas.96.3.1077
发表时间：
1999-02-02
影响因子：
11.1
作者：
Dawid, S;Barenkamp, SJ;St Geme, JW
通讯作者：
St Geme, JW
影响因子：
4.4
作者：
Hallstrom, Teresia;Trajkovska, Elena;Riesbeck, Kristian
通讯作者：
Riesbeck, Kristian
影响因子：
2.1
作者：
Morton, DJ;Stull, TL
通讯作者：
Stull, TL