A simple check valve for microfluidic point of care diagnostics.
A simple check valve for microfluidic point of care diagnostics.
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DOI：
10.1039/c6lc01104g
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发表时间：
2016-11-01
期刊：
影响因子：
6.1
通讯作者：
Meagher RJ
中科院分区：
文献类型：
--
作者：
Ball CS;Renzi RF;Priye A;Meagher RJ
止回阀通常是微流体设备中的重要组件，可在护理点实现自动化样本处理以进行诊断。然而，在开发的所有阶段--从用激光切割机的初始原型设计到用注塑成型的最终生产--都需要一种与刚性热塑性器件兼容的止回阀设计。在这里，我们介绍了一种被动式常闭止回阀的简单设计，该止回阀由常用材料制成，采用CO2激光器，易于集成到原型和生产热塑性器件中。止回阀由一个热塑性平面弹簧和一个柔软的弹性垫组成，它们共同作用以密封流体回流。阀门的开启压力可以通过改变弹簧的平面几何形状和厚度来调整。在弹性垫下方增加一个凸起的环形凸台，可改善密封完整性。为了证明阀门的有用性，我们采用这些阀门来创建一个手指操作的芯片上的试剂水库和手指驱动的气动泵。我们还应用这种止回阀来被动地密封设备，从而能够在激光切割设备中便携式地检测西尼罗河病毒的RNA。激光切割微流体止回阀可实现分阶段试剂输送、泵送和现场核酸扩增检测。
Check valves are often essential components in microfluidic devices, enabling automated sample processing for diagnostics at the point of care. However, there is an unmet need for a check valve design that is compatible with rigid thermoplastic devices during all stages of development—from initial prototyping with a laser cutter to final production with injection molding. Here, we present simple designs for a passive, normally closed check valve that is manufactured from commonly available materials with a CO2 laser and readily integrated into prototype and production thermoplastic devices. The check valve consists of a thermoplastic planar spring and a soft elastomeric pad that act together to seal against fluid backflow. The valve’s cracking pressure can be tuned by modifying the spring’s planar geometry and thickness. Seal integrity is improved with the addition of a raised annular boss beneath the elastomeric pad. To demonstrate the valve’s usefulness, we employ these valves to create a finger-operated on-chip reagent reservoir and a finger-actuated pneumatic pump. We also apply this check valve to passively seal a device to enable portable detection of RNA from West Nile virus in a laser-cut device. Laser cut microfluidic check valves enable staged reagent delivery, pumping, and point of care nucleic acid amplification testing.
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影响因子：
9.4
作者：
Parida, M;Posadas, G;Morita, K
通讯作者：
Morita, K
挤压芯片：手指控制的微流体网络装置及其在生化分析中的应用
影响因子：
6.1
作者：
Li, Wentao;Chen, Tao;Huang, Yanyi
通讯作者：
Huang, Yanyi
影响因子：
2.3
作者：
Smal, Olivier;Dehez, Bruno;Puers, Robert
通讯作者：
Puers, Robert
影响因子：
2.8
作者：
Snakenborg, Detlef;Klank, Henning;Kutter, Jorg P.
通讯作者：
Kutter, Jorg P.
影响因子：
6.1
作者：
Fong, Jeffrey;Xiao, Zhiming;Takahata, Kenichi
通讯作者：
Takahata, Kenichi