Transcriptional Landscapes of Long Non-coding RNAs and Alternative Splicing in Pyricularia oryzae Revealed by RNA-Seq.
Transcriptional Landscapes of Long Non-coding RNAs and Alternative Splicing in Pyricularia oryzae Revealed by RNA-Seq.
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RNA-Seq 揭示米瘟病菌中长非编码 RNA 的转录景观和选择性剪接
DOI：
10.3389/fpls.2021.723636
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发表时间：
2021
影响因子：
5.6
通讯作者：
Peng YL
中科院分区：
文献类型：
--
作者：
Li Z;Yang J;Peng J;Cheng Z;Liu X;Zhang Z;Bhadauria V;Zhao W;Peng YL
稻瘟病引起稻瘟病，这是世界上最具破坏性的作物病害之一，是一种模型真菌病原体，广泛用于剖析真菌毒力/致病性的分子机制。尽管米霉的全基因组序列是公开的，但其目前的转录组仍然不完整，缺乏非蛋白质编码基因和选择性剪接的信息。在这里，我们对分生孢子和菌丝进行了 RNA-Seq 分析，鉴定出了 3,374 个新基因。有趣的是，这些新基因中的绝大多数可能转录长非编码RNA（lncRNA），并且大多数位于基因间区域。值得注意的是，它们的表达与其邻近基因在分生孢子和菌丝中的转录同时进行。此外，还发现 2,358 个基因发生选择性剪接事件。此外，我们举例说明了 lncRNA 对菌丝生长很重要，可能是通过调节邻近的蛋白质编码基因，并且转录因子基因 CON7 的选择性剪接是附着胞形成所必需的。总之，本研究结果表明，lncRNA转录本和选择性剪接事件是调节对分生孢子、菌丝生长和发病机制重要的基因表达的两个重要机制，并为稻瘟病菌转录组和基因调控提供了新的见解。
Pyricularia oryzae causes the rice blast, which is one of the most devastating crop diseases worldwide, and is a model fungal pathogen widely used for dissecting the molecular mechanisms underlying fungal virulence/pathogenicity. Although the whole genome sequence of P. oryzae is publicly available, its current transcriptomes remain incomplete, lacking the information on non-protein coding genes and alternative splicing. Here, we performed and analyzed RNA-Seq of conidia and hyphae, resulting in the identification of 3,374 novel genes. Interestingly, the vast majority of these novel genes likely transcribed long non-coding RNAs (lncRNAs), and most of them were localized in the intergenic regions. Notably, their expressions were concomitant with the transcription of neighboring genes thereof in conidia and hyphae. In addition, 2,358 genes were found to undergo alternative splicing events. Furthermore, we exemplified that a lncRNA was important for hyphal growth likely by regulating the neighboring protein-coding gene and that alternative splicing of the transcription factor gene CON7 was required for appressorium formation. In summary, results from this study indicate that lncRNA transcripts and alternative splicing events are two important mechanisms for regulating the expression of genes important for conidiation, hyphal growth, and pathogenesis, and provide new insights into transcriptomes and gene regulation in the rice blast fungus.
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影响因子：
14.9
作者：
Kanehisa M;Goto S;Sato Y;Furumichi M;Tanabe M
通讯作者：
Tanabe M
影响因子：
6.7
作者：
Kong LA;Yang J;Li GT;Qi LL;Zhang YJ;Wang CF;Zhao WS;Xu JR;Peng YL
通讯作者：
Peng YL
Wolf Psort：蛋白质定位预测因子。
影响因子：
14.9
作者：
Horton, Paul;Park, Keun-Joon;Obayashi, Takeshi;Fujita, Naoya;Harada, Hajime;Adams-Collier, C J;Nakai, Kenta
通讯作者：
Nakai, Kenta
影响因子：
3
作者：
Kong, Ling-An;Li, Guo-Tian;Xu, Jin-Rong
通讯作者：
Xu, Jin-Rong
影响因子：
56.9
作者：
Heo, Jae Bok;Sung, Sibum
通讯作者：
Sung, Sibum