CHROMOMETHYLTRANSFERASE3/KRYPTONITE maintains the sulfurea paramutation in Solanum lycopersicum.
CHROMOMETHYLTRANSFERASE3/KRYPTONITE maintains the sulfurea paramutation in Solanum lycopersicum.
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色甲基转移酶3/KRYPTONITE维持番茄中的硫脲副突变。
DOI：
10.1073/pnas.2112240119
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发表时间：
2022-03-29
影响因子：
11.1
通讯作者：
中科院分区：
文献类型：
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作者：
副突变涉及沉默和活跃等位基因之间抑制性表观遗传标记的转移。它最为人所知的是在玉米中以及在其他植物和动物中的显着表型的特殊非孟德尔遗传。然而，最近的基因组研究表明，副突变可能不那么例外。它可能是远缘杂交的结果，并可能导致作物的数量性状变异或不稳定的表型。使用的sulfurea（sulf）在番茄中的轨迹，我们表明，一个自我加强的反馈回路，涉及DNA和组蛋白甲基转移酶3（CMT 3）和KRYPTONITE（KYP）是所需的paramutation的sulf和染色质组织的变化。这些发现促进了对植物非孟德尔遗传的理解。副突变涉及到一个抑制性表观遗传标记从沉默等位基因转移到一个活跃的同系物，因此，非孟德尔遗传。在番茄中，sulfurea（sulf）paramutation与高水平的CHG超甲基化的区域重叠的转录起始位点（TSS）的SlTAB 2基因，影响叶绿素合成。相对于CTG或CAG，CCG亚背景超甲基化在该区域代表性不足，暗示了该基因座处的染色体甲基转移酶3a（CMT 3）的副突变。与这种解释一致，CMT 3功能的丧失导致硫失绿症、相关的CHG超甲基化和副突变的丧失。KRYPTONITE（KYP）组蛋白甲基转移酶功能的丧失具有与SlTAB 2的启动子区域处的H3 K9 me 2减少和该基因座处的高阶染色质结构的移位相关的类似效应。相反，RNA聚合酶V（PolV）的最大亚基的突变不影响sulf paramutation。这些发现表明，在副突变的维持中，CMT 3/KYP依赖性反馈环而不是导致RNA指导的DNA甲基化（RdDM）的PolV依赖性途径的参与。
Paramutation involves the transfer of a repressive epigenetic mark between silent and active alleles. It is best known from exceptional non-Mendelian inheritance of conspicuous phenotypes in maize but also in other plants and animals. Recent genomic studies, however, indicate that paramutation may be less exceptional. It may be a consequence of wide-cross hybridization and may contribute to quantitative trait variation or unstable phenotypes in crops. Using the sulfurea (sulf) locus in tomato, we demonstrate that a self-reinforcing feedback loop involving DNA- and histone-methyl transferases CHROMOMETHYLTRANSFERASE3 (CMT3) and KRYPTONITE (KYP) is required for paramutation of sulf and that there is a change in chromatin organization. These findings advance the understanding of non-Mendelian inheritance in plants. Paramutation involves the transfer of a repressive epigenetic mark from a silent allele to an active homolog and, consequently, non-Mendelian inheritance. In tomato, the sulfurea (sulf) paramutation is associated with a high level of CHG hypermethylation in a region overlapping with the transcription start site (TSS) of the SlTAB2 gene that affects chlorophyll synthesis. The CCG subcontext hypermethylation is under-represented at this region relative to CTG or CAG, implicating the CHROMOMETHYLTRANSFERASE3a (CMT3) in paramutation at this locus. Consistent with this interpretation, loss of CMT3 function leads to loss of the sulf chlorosis, the associated CHG hypermethylation, and paramutation. Loss of KRYPTONITE (KYP) histone methyltransferase function has a similar effect linked to reduced H3K9me2 at the promoter region of SlTAB2 and a shift in higher order chromatin structure at this locus. Mutation of the largest subunit of RNA polymerase V (PolV) in contrast does not affect sulf paramutation. These findings indicate the involvement of a CMT3/KYP–dependent feedback loop rather than the PolV-dependent pathway leading to RNA-directed DNA methylation (RdDM) in the maintenance of paramutation.
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影响因子：
4.5
作者：
Sekhon, Rajandeep S.;Wang, Po-Hao;Chopra, Surinder
通讯作者：
Chopra, Surinder
植物铬甲基转移酶的DNA甲基化特征。
影响因子：
4.5
作者：
Gouil Q;Baulcombe DC
通讯作者：
Baulcombe DC
DOI：
10.1098/rspb.1920.0009
发表时间：
1920-05-01
期刊：
PROCEEDINGS OF THE ROYAL SOCIETY OF LONDON SERIES B-CONTAINING PAPERS OF A BIOLOGICAL CHARACTER
影响因子：
--
作者：
Bateson, W;Pellew, C
通讯作者：
Pellew, C
影响因子：
5.4
作者：
HAGEMANN, R
通讯作者：
HAGEMANN, R
影响因子：
7.2
作者：
Haring, Max;Bader, Rechien;Stam, Maike
通讯作者：
Stam, Maike