NaCl potentiates human fibrocyte differentiation.
NaCl potentiates human fibrocyte differentiation.
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DOI：
10.1371/journal.pone.0045674
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发表时间：
2012
期刊：
影响因子：
3.7
通讯作者：
Gomer RH
中科院分区：
文献类型：
--
作者：
Cox N;Pilling D;Gomer RH
过量的氯化钠摄入与多种纤维化疾病有关，例如肾脏和心脏纤维化。这种关联归因于高氯化钠摄入导致血压升高。然而，对高氯化钠摄入和纤维化患者的研究表明，氯化钠摄入和纤维化之间存在独立于血压的联系。我们发现，在无血清培养条件下，将细胞外的 NaCl 浓度提高到高盐摄入后人血液中可能出现的水平，可以增强新分离的人单核细胞向称为纤维细胞的成纤维细胞样细胞的分化。氯化钠在单核细胞粘附过程中直接影响单核细胞。氯化钾和硝酸钠也能增强纤维细胞的分化。血浆蛋白血清淀粉样蛋白 P (SAP) 抑制纤维细胞分化。高浓度的细胞外 NaCl 使 SAP Hill 系数从 1.7 变为 0.8，并导致抑制纤维细胞分化 95% 所需的 SAP 浓度增加四倍。总之，我们的数据表明 NaCl 增强纤维细胞分化。因此，氯化钠增加的纤维细胞分化可能导致氯化钠增加的肾脏和心脏纤维化。
Excessive NaCl intake is associated with a variety of fibrosing diseases such as renal and cardiac fibrosis. This association has been attributed to increased blood pressure as the result of high NaCl intake. However, studies in patients with high NaCl intake and fibrosis reveal a connection between NaCl intake and fibrosis that is independent of blood pressure. We find that increasing the extracellular concentration of NaCl to levels that may occur in human blood after high-salt intake can potentiate, in serum-free culture conditions, the differentiation of freshly-isolated human monocytes into fibroblast-like cells called fibrocytes. NaCl affects the monocytes directly during their adhesion. Potassium chloride and sodium nitrate also potentiate fibrocyte differentiation. The plasma protein Serum Amyloid P (SAP) inhibits fibrocyte differentiation. High levels of extracellular NaCl change the SAP Hill coefficient from 1.7 to 0.8, and cause a four-fold increase in the concentration of SAP needed to inhibit fibrocyte differentiation by 95%. Together, our data suggest that NaCl potentiates fibrocyte differentiation. NaCl-increased fibrocyte differentiation may thus contribute to NaCl-increased renal and cardiac fibrosis.
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影响因子：
3.3
作者：
Farquhar, WB;Paul, EE;Stillabower, ME
通讯作者：
Stillabower, ME
影响因子：
5.6
作者：
HAMILTON, JA;STANLEY, ER;SHADDUCK, RK
通讯作者：
SHADDUCK, RK
影响因子：
--
作者：
FROHLICH, ED;CHIEN, YW;PEGRAM, BL
通讯作者：
PEGRAM, BL
DOI：
10.1073/pnas.0608799103
发表时间：
2006-11-28
影响因子：
11.1
作者：
Haudek, Sandra B.;Xia, Ying;Entman, Mark L.
通讯作者：
Entman, Mark L.
DOI：
10.1111/j.1524-475x.2009.00482.x
发表时间：
2009-05
期刊：
Wound repair and regeneration : official publication of the Wound Healing Society [and] the European Tissue Repair Society
影响因子：
--
作者：
Gomer RH;Pilling D;Kauvar LM;Ellsworth S;Ronkainen SD;Roife D;Davis SC
通讯作者：
Davis SC