RNA editing and alternative splicing of the insect nAChR subunit alpha6 transcript: evolutionary conservation, divergence and regulation.
RNA editing and alternative splicing of the insect nAChR subunit alpha6 transcript: evolutionary conservation, divergence and regulation.
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DOI：
10.1186/1471-2148-7-98
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发表时间：
2007-06-27
影响因子：
3.4
通讯作者：
Lv J
中科院分区：
文献类型：
--
作者：
Jin Y;Tian N;Cao J;Liang J;Yang Z;Lv J
RNA编辑和选择性剪接在扩大蛋白质多样性中起着重要作用，这在烟碱乙酰胆碱受体（nAChR）的研究中得到了很好的说明。在这里，我们比较了来自不同昆虫的nAChR α 6亚基基因的RNA编辑和选择性剪接，这些昆虫跨越了约3亿年的进化--果蝇、冈比亚按蚊、家蚕、赤拟谷盗和意大利蜜蜂。保守的和物种特异性的A-to-I RNA编辑发生在除A.冈比亚，显示非常短的侧翼内含子序列。有趣的是，在其他物种中，一些A-to-I编辑位点是基因组编码的G。实验数据和计算分析不同物种的orthopathy alpha 6基因的组合表明，RNA编辑和选择性剪接至少早于跨越约3亿年进化的昆虫目的辐射;然而，它们可能在随后的进化过程中在某些物种中丢失。发现选择性剪接的发生以不同的模式进行调节，在某些情况下，甚至与RNA编辑相关。基于对3亿年进化历史中不同昆虫的orthogonnAChR α 6基因的比较分析，我们记录了RNA编辑和选择性剪接的存在、进化保守性和差异性，以及调控。RNA编辑和选择性剪接的系统发育分析可以产生多种功能不同的蛋白质同种型，可能在核苷酸和蛋白质序列之外的复杂生物体的进化中发挥关键作用。
RNA editing and alternative splicing play an important role in expanding protein diversity and this is well illustrated in studies of nicotinic acetylcholine receptors (nAChRs). Here, we compare the RNA editing and alternative splicing of the nAChR alpha6 subunit genes from different insects spanning ~300 million years of evolution– Drosophila melanogaster, Anopheles gambiae, Bombyx mori, Tribolium castaneum and Apis mellifera. The conserved and species-specific A-to-I RNA editing occurred across all species except A. gambiae, which displayed extraordinarily short flanking intronic sequences. Interestingly, some A-to-I editing sites were a genomically encoded G in other species. A combination of the experimental data and computational analysis of orthologous alpha6 genes from different species indicated that RNA editing and alternative splicing predated at least the radiation of insect orders spanning ~300 million years of evolution; however, they might have been lost in some species during subsequent evolution. The occurrence of alternative splicing was found to be regulated in distinct modes and, in some cases, even correlated with RNA editing. On the basis of comparative analysis of orthologous nAChR alpha6 genes from different insects spanning ~300 million years of evolution, we have documented the existence, evolutionary conservation and divergence, and also regulation of RNA editing and alternative splicing. Phylogenetic analysis of RNA editing and alternative splicing, which can create a multitude of functionally distinct protein isoforms, might have a crucial role in the evolution of complex organisms beyond nucleotide and protein sequences.
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影响因子：
14.9
作者：
Cartegni, L;Wang, JH;Krainer, AR
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