Development of an Enzyme-Linked Immunosorbent Assay and Gold-Labelled Immunochromatographic Strip Assay for the Detection of Ancient Wool.
Development of an Enzyme-Linked Immunosorbent Assay and Gold-Labelled Immunochromatographic Strip Assay for the Detection of Ancient Wool.
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开发用于检测古代羊毛的酶联免疫吸附测定法和金标免疫层析试纸条测定法
DOI：
10.1155/2018/2641624
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发表时间：
2018
影响因子：
2.6
通讯作者：
Hu Z
中科院分区：
文献类型：
--
作者：
Wang B;Gu J;Chen B;Xu C;Zheng H;Peng Z;Zhou Y;Hu Z
The identification of ancient wool is of great importance in archaeology.尽管通过传统的检测方法，即光学和电子显微镜、光谱学和色谱法，可以获得许多有意义的信息，但在检测污染或严重降解的古代样品时，其功效可能会受到限制。 In this work, an immunoassay was proposed and performed for the identification of ancient wool.首先，通过完全抗原（角蛋白）免疫家兔直接制备特异性抗体，成本低、操作简便、适用范围广。然后，开发了酶联免疫吸附测定（ELISA）和胶体金标记免疫层析条带（ICS）来定性鉴定哈萨克斯坦和中国出土的古代羊毛样品中的相应蛋白质。 The anti-keratin antibody exhibited high sensitivity and specificity for the identification of modern and ancient wool. The limit of detection (LOD) of the ELISA method was 10 ng/mL, and no cross-reactions with other interfering antigens have been noted. It is concluded that the immunoassays are reliable methods for the identification of ancient wool.
The identification of ancient wool is of great importance in archaeology. Despite lots of meaningful information can be achieved by conventional detection methods, that is, light and electron microscopy, spectroscopy, and chromatography, the efficacy is likely to be limited in the detection of ancient samples with contamination or severe degradation. In this work, an immunoassay was proposed and performed for the identification of ancient wool. First, a specific antibody, which has the benefits of low cost, easy operation, and extensive applicability, was developed directly through immunizing rabbits with complete antigen (keratin). Then, an enzyme-linked immunosorbent assay (ELISA) and a colloidal gold-labelled immunochromatographic strip (ICS) were developed to qualitatively identify the corresponding protein in ancient wool samples unearthed from Kazakhstan and China. The anti-keratin antibody exhibited high sensitivity and specificity for the identification of modern and ancient wool. The limit of detection (LOD) of the ELISA method was 10 ng/mL, and no cross-reactions with other interfering antigens have been noted. It is concluded that the immunoassays are reliable methods for the identification of ancient wool.
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影响因子：
3.8
作者：
Cardamone, Jeanette M.
通讯作者：
Cardamone, Jeanette M.
影响因子：
3.3
作者：
Clerens, Stefan;Cornellison, Charisa D.;Dyer, Jolon M.
通讯作者：
Dyer, Jolon M.
影响因子：
3.8
作者：
Wojciechowska, E;Rom, M;Weselucha-Birczynska, A
通讯作者：
Weselucha-Birczynska, A
影响因子：
3.5
作者：
Bruni, Silvia;De Luca, Eleonora;Pozzi, Federica
通讯作者：
Pozzi, Federica
DOI：
10.1179/019713606806112522
发表时间：
2006-06-01
影响因子：
0.5
作者：
Heginbotham, Arlen;Millay, Victoria;Quick, Michael
通讯作者：
Quick, Michael