In situ hybridization is a necessary experimental complement to microRNA (miRNA) expression profiling in the human brain.
In situ hybridization is a necessary experimental complement to microRNA (miRNA) expression profiling in the human brain.
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DOI：
10.1016/j.neulet.2009.04.044
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发表时间：
2009-12-04
影响因子：
2.5
通讯作者：
Wilfred BR
中科院分区：
文献类型：
--
作者：
Nelson PT;Wilfred BR
microRNAs（miRNAs）在人脑神经化学中起着重要作用。然而，在这个快节奏的领域还有很多东西要学。为了了解miRNAs如何促进正常的生物学功能和疾病状态，了解在一系列条件下在特定细胞类型中表达的miRNAs至关重要。已经开发了许多工具来帮助描述给定样品中存在于组织水平的miRNA库。然而，组织水平的miRNA谱不足以精确定位单个miRNA的细胞和亚细胞分布。这种知识在神经系统中尤其重要，因为神经系统具有许多细胞类型、功能不同的细胞群的微观异质性以及细胞形状的极端几何复杂性。我们已经发现，原位杂交显示了重要的大脑皮层特异性的miRNA表达模式，这将在大多数组织水平的表达研究中丢失，并且这些特异性的模式可以直接与人脑疾病相关。因此，原位杂交是组织水平miRNA表达谱的重要实验补充。描述了这项重要技术的技术和理论方面，特别是与研究人脑有关的技术。
MicroRNAs (miRNAs) play fundamental roles in human brain neurochemistry. However, much remains to be learned in this fast-paced field. To understand how miRNAs contribute to normal biologic functions and disease states, it is critical to understand the miRNAs that are expressed in particular cell types under a range of conditions. Many tools have been developed to help describe the repertoire of miRNAs present at the tissue level in a given sample. However, tissue level miRNA profiling is inadequate to pinpoint the cellular and sub-cellular distribution of individual miRNAs. Such knowledge is especially important in the nervous system with its many cell types, microscopic heterogeneity with regard to functionally distinct cell groups, and extreme geometrical complexity in cellular shapes. We have found that in situ hybridization shows important cerebral cortical lamina-specific patterns of miRNA expression that would be lost on most tissue level expression studies, and these lamina-specific patterns can be directly relevant to human brain disease. Thus, in situ hybridization is an important experimental complement to tissue-level miRNA expression profiling. Technical and theoretical aspects of this important technique are described, especially those pertinent to studying the human brain.
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影响因子：
56.9
作者：
Kim, Jongpil;Inoue, Keiichi;Abeliovich, Asa
通讯作者：
Abeliovich, Asa
影响因子：
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作者：
Krichevsky, Anna M.;Sonntag, Kai-C.;Kosik, Kenneth S.
通讯作者：
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影响因子：
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作者：
Martienssen, RA
通讯作者：
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影响因子：
6.4
作者：
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通讯作者：
Rajeev, Bernard W.
DOI：
10.1073/pnas.0710263105
发表时间：
2008-04-29
影响因子：
11.1
作者：
Hebert, Sebastien S.;Horre, Katrien;De Strooper, Bart
通讯作者：
De Strooper, Bart