Composition and structure of photosystem I in the moss Physcomitrella patens.

Composition and structure of photosystem I in the moss Physcomitrella patens.
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DOI:
10.1093/jxb/ert126
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发表时间:
2013-07
影响因子:
6.9
通讯作者:
Jensen PE
Jensen PE
中科院分区:
生物学1区
文献类型:
--
作者:
Busch A;Petersen J;Webber-Birungi MT;Powikrowska M;Lassen LM;Naumann-Busch B;Nielsen AZ;Ye J;Boekema EJ;Jensen ON;Lunde C;Jensen PE

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近年来,从4亿多年前种子植物祖先分化而来的苔藓植物成为光合作用研究的热点,因为它们可以为陆地生物适应过程中光合作用复合体的进化提供有价值的见解。本研究从青海苔藓中分离出完整的光系统I(PSI)及其捕光复合体(LHCI),并利用电子显微镜、质谱学、生化和生理学等方法对其结构、多肽组成和捕光功能进行了研究。很明显,Physcomitrella对不同的PSI核心亚基和LHCI蛋白都有非常高的异构体数量。结果表明,所有这些不同的亚基异构体都在蛋白质水平上表达,并被整合到功能性的PSI-LHCI复合体中。此外,与以往的报道不同的是,研究表明,Physcomitrella组装了一个由四个捕光蛋白组成的捕光复合体,形成了一个更高级的植物样PSI超结构。
Recently, bryophytes, which diverged from the ancestor of seed plants more than 400 million years ago, came into focus in photosynthesis research as they can provide valuable insights into the evolution of photosynthetic complexes during the adaptation to terrestrial life. This study isolated intact photosystem I (PSI) with its associated light-harvesting complex (LHCI) from the moss Physcomitrella patens and characterized its structure, polypeptide composition, and light-harvesting function using electron microscopy, mass spectrometry, biochemical, and physiological methods. It became evident that Physcomitrella possesses a strikingly high number of isoforms for the different PSI core subunits as well as LHCI proteins. It was demonstrated that all these different subunit isoforms are expressed at the protein level and are incorporated into functional PSI–LHCI complexes. Furthermore, in contrast to previous reports, it was demonstrated that Physcomitrella assembles a light-harvesting complex consisting of four light-harvesting proteins forming a higher-plant-like PSI superstructure.
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