Study on complications of osteoporosis based on network pharmacology.
Study on complications of osteoporosis based on network pharmacology.
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基于网络药理学的骨质疏松症并发症研究。
DOI：
10.3389/fgene.2022.941098
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发表时间：
2022
影响因子：
3.7
通讯作者：
Wang, Wei
中科院分区：
文献类型：
--
作者：
Song, Zhijing;Zhang, Haoling;Jiang, Yuhang;Zhao, Rui;Pei, Xuedong;Ning, Haochi;Chen, Hailiang;Pan, Jing;Gong, Yanlong;Song, Min;Wang, Wei
关键词：
骨质疏松症严重威胁人类生命。固本增骨颗粒是临床治疗骨质疏松症的经验方。 MC3T3-E1细胞是具有成骨分化能力的小鼠成骨前体细胞，是研究骨代谢和成骨机制的经典细胞，也是机械刺激敏感细胞。由此推测，固本增骨颗粒可以修复持续静压超负荷下的MC3T3-E1细胞。本研究旨在通过网络药理学和基因测序方法，揭示持续静压超负荷条件下节俭增加骨颗粒对MC3T3-E1细胞成骨的影响机制。在分析过程中，从数据库中预测了多种98种化合物，收集了474个目标，两组基因之间共有29,164个差异。然后，构建细胞间复合靶点和预测靶点以及蛋白质-蛋白质相互作用网络，并通过聚类分析进一步探讨靶点之间的关系。此外，利用基因本体论（GO）和通路（KEGG Pathway）进一步鉴定了靶蛋白和细胞之间的联系。最后通过实验验证固本增骨颗粒对持续静压超负荷下MC3T3-E1细胞的修复作用，从而准确解释中药药效机制。
Osteoporosis is a serious threat to human life. Guben Zenggu Granule is an empirical prescription for clinical treatment of osteoporosis. MC3T3-E1 cells are mouse osteogenic precursor cells with osteogenic differentiation, and are classic cells for studying bone metabolism and osteogenic mechanism, as well as mechanical stimulation sensitive cells. Therefore, it can be inferred that Guben Zenggu granule can repair MC3T3-E1 cells under continuous static pressure overload. This study aims to through the network of pharmacology and gene sequencing method, reveal thrift increase bone particles under the condition of continuous static pressure overload on osteogenesis mechanism of MC3T3-E1 cells. In the process of analysis, from a variety of 98 compounds was predicted in the database, a collection of 474 goals, a total of 29,164 difference between two groups of genes. Then, construction of composite targets between cells and predict targets and protein - protein interaction networks, and through the cluster analysis to further explore the relationship between the target. In addition, linkages between target proteins and cells were further identified using Gene Ontology (GO) and Pathways (KEGG Pathway). Finally, the repair effect of Guben Zenggu granule on MC3T3-E1 cells under continuous static pressure overload was verified through experiments, so as to accurately explain the pharmacodynamic mechanism of Traditional Chinese medicine.
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影响因子：
3.8
作者：
通讯作者：
--
网络药理学方法确定葛根芩连汤治疗2型糖尿病的活性成分和作用机制。
DOI：
10.1155/2014/495840
发表时间：
2014
期刊：
Evidence-based complementary and alternative medicine : eCAM
影响因子：
--
作者：
Li H;Zhao L;Zhang B;Jiang Y;Wang X;Guo Y;Liu H;Li S;Tong X
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Tong X
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影响因子：
4.6
作者：
Liu Z;Guo F;Wang Y;Li C;Zhang X;Li H;Diao L;Gu J;Wang W;Li D;He F
通讯作者：
He F
影响因子：
4
作者：
Kanis, J. A.;Cooper, C.;Reginster, J. -Y.
通讯作者：
Reginster, J. -Y.
DOI：
10.1016/j.injury.2017.08.036
发表时间：
2017-12-01
影响因子：
2.5
作者：
Yaacobi, Eyal;Sanchez, Daniela;Horwitz, Daniel S.
通讯作者：
Horwitz, Daniel S.