Microbial communities exhibit host species distinguishability and phylosymbiosis along the length of the gastrointestinal tract.
Microbial communities exhibit host species distinguishability and phylosymbiosis along the length of the gastrointestinal tract.
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DOI：
10.1111/mec.14460
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发表时间：
2018-04
影响因子：
4.9
通讯作者：
Bordenstein SR
中科院分区：
文献类型：
--
作者：
Kohl KD;Dearing MD;Bordenstein SR
宿主相关的微生物群落由稳定的和短暂的成员组成，这些成员可以通过与环境相关的纯随机过程或与宿主的相互作用来聚集。系统共生预测，如果宿主-微生物群相互作用影响组装模式，那么一个可能的结果是宿主进化历史（系统发育）和微生物群落结构（微生物群树形图）的生态相似性之间的一致性。这种组装模式已在实验室和自然种群的动物宿主的几个分支中得到证实，但在脊椎动物中，仅使用粪便或远端结肠样本进行了研究。在这里，我们收集了7种啮齿类动物的5个肠道区域的内容物，并通过16S rRNA基因测序对细菌群落进行了盘点。我们研究了群落结构如何在肠道区域变化，以及沿肠道长度是否存在系统共生模式。肠道菌群因宿主种类和肠道区域而异，其中示波螺旋菌和瘤胃球菌在胃和后肠区域更为丰富。肠道微生物群落在所有肠道区域被宿主物种高度区分，并且随着肠道长度的增加，区分的强度增加。最后，在所有五个肠道区域以及粪便中都发现了系统共生模式。肠道环境的各个方面，如氧气水平、抗菌剂的产生或其他因素可能会改变肠道区域的微生物群落。然而，尽管存在这些差异，宿主物种保持着可区分的、系统共生的微生物组合，这些微生物可能对宿主产生功能影响。
Host-associated microbial communities consist of stable and transient members that can assemble through purely stochastic processes associated with the environment or by interactions with the host. Phylosymbiosis predicts that if host-microbiota interactions impact assembly patterns, then one conceivable outcome is concordance between host evolutionary histories (phylogeny) and the ecological similarities in microbial community structures (microbiota dendrogram). This assembly pattern has been demonstrated in several clades of animal hosts in laboratory and natural populations, but in vertebrates it has only been investigated using samples from feces or the distal colon. Here, we collected the contents of five gut regions from seven rodent species and inventoried the bacterial communities by sequencing the 16S rRNA gene. We investigated how community structures varied across gut regions and whether the pattern of phylosymbiosis was present along the length of the gut. Gut communities varied by host species and gut region, with Oscillospira and Ruminococcus being more abundant in the stomach and hindgut regions. Gut microbial communities were highly distinguishable by host species across all gut regions, with the strength of the discrimination increasing along the length of the gut. Last, the pattern of phylosymbiosis was found in all five gut regions, as well as feces. Aspects of the gut environment, such as oxygen levels, production of antimicrobials, or other factors may shift microbial communities across gut regions. However, regardless of these differences, host species maintain distinguishable, phylosymbiotic assemblages of microbes that may have functional impacts for the host.
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DOI：
10.1038/nrmicro3552
发表时间：
2016-01
期刊：
Nature reviews. Microbiology
影响因子：
--
作者：
Donaldson GP;Lee SM;Mazmanian SK
通讯作者：
Mazmanian SK
影响因子：
9.8
作者：
Brooks AW;Kohl KD;Brucker RM;van Opstal EJ;Bordenstein SR
通讯作者：
Bordenstein SR
DOI：
10.1038/ismej.2012.8
发表时间：
2012-08
期刊：
The ISME journal
影响因子：
--
作者：
通讯作者：
--
DOI：
10.1126/science.1224203
发表时间：
2012-06-08
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Costello EK;Stagaman K;Dethlefsen L;Bohannan BJ;Relman DA
通讯作者：
Relman DA
影响因子：
9.8
作者：
Bordenstein SR;Theis KR
通讯作者：
Theis KR