Sialic acid-dependent interactions between influenza viruses and Streptococcus suis affect the infection of porcine tracheal cells.
Sialic acid-dependent interactions between influenza viruses and Streptococcus suis affect the infection of porcine tracheal cells.
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流感病毒和猪链球菌之间唾液酸依赖性相互作用影响猪气管细胞的感染
DOI：
10.1099/jgv.0.000223
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发表时间：
2015
期刊：
影响因子：
--
通讯作者：
Herrler G
中科院分区：
文献类型：
--
作者：
Meng F;Seitz M;Valentin-Weigand P;Herrler G
细菌合并感染是流感病毒引起的人类和动物疾病的主要并发症。任何一种病原体都可以诱导宿主反应，从而影响另一种病原体的感染。猪流感病毒（SIV）和猪链球菌2型（Streptococcus suisserotype 2）混合感染的一个独特特征是两种病原体之间的直接相互作用。由SIV的血凝素介导，该血凝素识别存在于猪链球菌荚膜多糖中的α 2，6-连接唾液酸。在本研究中，H1N1和H3 N2亚型的SIV以及识别α 2，6-连接唾液酸的人流感病毒都证明了这种相互作用。SIV与猪链球菌的结合导致病毒与细菌在低速离心时共沉淀。与对照病毒相比，与细菌结合的病毒保留了感染性，但仅诱导了微小的噬斑。SIV感染猪气管细胞可促进猪链球菌的粘附，细菌和病毒抗原的共染色证明了这一点。孵育30分钟后，已经可以检测到猪链球菌的唾液酸依赖性结合。 相比之下，细菌共感染对SIV的复制具有负面影响，如上清液中较低的病毒滴度和病毒释放动力学的延迟所示。
Bacterial co-infections are a major complication in influenza-virus-induced disease in both humans and animals. Either of the pathogens may induce a host response that affects the infection by the other pathogen. A unique feature in the co-infection by swine influenza viruses (SIV) andStreptococcus suisserotype 2 is the direct interaction between the two pathogens. It is mediated by the haemagglutinin of SIV that recognizes the α2,6-linked sialic acid present in the capsular polysaccharide ofStreptococcus suis. In the present study, this interaction was demonstrated for SIV of both H1N1 and H3N2 subtypes as well as for human influenza viruses that recognize α2,6-linked sialic acid. Binding of SIV toStreptococcus suisresulted in co-sedimentation of virus with bacteria during low-speed centrifugation. Viruses bound to bacteria retained infectivity but induced only tiny plaques compared with control virus. Infection of porcine tracheal cells by SIV facilitated adherence ofStreptococcus suis, which was evident by co-staining of bacterial and viral antigen. Sialic-acid-dependent binding ofStreptococcus suiswas already detectable after incubation for 30 min. By contrast, bacterial co-infection had a negative effect on the replication of SIV as indicated by lower virus titres in the supernatant and a delay in the kinetics of virus release.
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影响因子：
30.3
作者：
Siegel SJ;Roche AM;Weiser JN
通讯作者：
Weiser JN
影响因子：
3.7
作者：
Sauer AK;Liang CH;Stech J;Peeters B;Quéré P;Schwegmann-Wessels C;Wu CY;Wong CH;Herrler G
通讯作者：
Herrler G
影响因子：
2.9
作者：
Van Calsteren, Marie-Rose;Gagnon, Fleur;Gottschalk, Marcello
通讯作者：
Gottschalk, Marcello
影响因子：
5.4
作者：
Leung, Horasis S. Y.;Li, Olive T. W.;Poon, Leo L. M.
通讯作者：
Poon, Leo L. M.
影响因子：
5.6
作者：
Stevens, J;Blixt, O;Wilson, IA
通讯作者：
Wilson, IA