Characterization of the sialic acid binding activity of influenza A viruses using soluble variants of the H7 and H9 hemagglutinins.
Characterization of the sialic acid binding activity of influenza A viruses using soluble variants of the H7 and H9 hemagglutinins.
复制标题
DOI：
10.1371/journal.pone.0089529
复制
发表时间：
2014
期刊：
影响因子：
3.7
通讯作者：
Herrler G
中科院分区：
文献类型：
--
作者：
Sauer AK;Liang CH;Stech J;Peeters B;Quéré P;Schwegmann-Wessels C;Wu CY;Wong CH;Herrler G
流感病毒与靶细胞的结合由病毒表面蛋白血凝素介导。为了确定细胞和组织上甲型流感病毒的结合位点的存在，通过将血凝素胞外域连接至人免疫球蛋白G的Fc部分（H7Fc和H9Fc）来产生H7和H9亚型的可溶性血凝素。这两种嵌合蛋白以唾液酸依赖性方式与不同的细胞和组织结合。在H7 Fc和H9 Fc与不同哺乳动物和禽类培养细胞的结合以及与不同病毒宿主物种（火鸡、鸡和猪）呼吸道上皮的冷冻切片的结合中观察到显著差异。可溶性血凝素的结合与病毒颗粒的结合相似，但与两种唾液酸特异性植物凝集素相比，结合模式存在差异。通过比较聚糖阵列分析证实了这些发现，该分析揭示了植物凝集素对唾液酸糖缀合物的非常窄的识别，其不反映优先被H7 Fc和H9 Fc识别的聚糖结构。因此，可溶性血凝素可以作为唾液酸特异性凝集素，并且是比常用的植物凝集素更可靠的流感病毒HA结合位点存在的指示剂。
Binding of influenza viruses to target cells is mediated by the viral surface protein hemagglutinin. To determine the presence of binding sites for influenza A viruses on cells and tissues, soluble hemagglutinins of the H7 and H9 subtype were generated by connecting the hemagglutinin ectodomain to the Fc portion of human immunoglobulin G (H7Fc and H9Fc). Both chimeric proteins bound to different cells and tissues in a sialic acid-dependent manner. Pronounced differences were observed between H7Fc and H9Fc, in the binding both to different mammalian and avian cultured cells and to cryosections of the respiratory epithelium of different virus host species (turkey, chicken and pig). Binding of the soluble hemagglutinins was similar to the binding of virus particles, but showed differences in the binding pattern when compared to two sialic acid-specific plant lectins. These findings were substantiated by a comparative glycan array analysis revealing a very narrow recognition of sialoglycoconjugates by the plant lectins that does not reflect the glycan structures preferentially recognized by H7Fc and H9Fc. Thus, soluble hemagglutinins may serve as sialic acid-specific lectins and are a more reliable indicator of the presence of binding sites for influenza virus HA than the commonly used plant lectins.
登录
查看更多内容
影响因子：
4.8
作者：
Pillai SP;Lee CW
通讯作者：
Lee CW
影响因子：
2.6
作者：
Nelli RK;Kuchipudi SV;White GA;Perez BB;Dunham SP;Chang KC
通讯作者：
Chang KC
影响因子：
3.7
作者：
Dong G;Luo J;Zhang H;Wang C;Duan M;Deliberto TJ;Nolte DL;Ji G;He H
通讯作者：
He H
影响因子：
3.8
作者：
Gohrbandt, Sandra;Veits, Jutta;Stech, Juergen
通讯作者：
Stech, Juergen
DOI：
10.1515/bchm2.1955.301.1-2.235
发表时间：
1955-01-01
影响因子：
--
作者：
KLENK, E;FAILLARD, H;LEMPFRID, H
通讯作者：
LEMPFRID, H