Identification and pyramiding of QTLs for cold tolerance at the bud bursting and the seedling stages by use of single segment substitution lines in rice (Oryza sativa L.)
Identification and pyramiding of QTLs for cold tolerance at the bud bursting and the seedling stages by use of single segment substitution lines in rice (Oryza sativa L.)
复制标题
利用单片段替代系对水稻 (Oryza sativa L.) 萌芽期和苗期耐寒性 QTL 进行鉴定和聚合
DOI：
10.1007/s11032-016-0520-9
复制
发表时间：
2016-07
影响因子：
3.1
通讯作者：
Liu Bin
中科院分区：
文献类型：
--
作者：
Yang Tifeng;Zhang Shaohong;Zhao Junliang;Liu Qing;Huang Zhanghui;Mao Xingxue;Dong Jingfang;Wang Xiaofei;Zhang Guiquan;Liu Bin
冷胁迫是制约水稻生产的主要因素之一，低温胁迫对水稻生长发育的影响可能发生在不同的发育阶段。了解耐寒性的遗传基础是选育耐寒品种的关键。本研究利用耐寒粳稻品种“南阳占”与籼稻品种“花粳鲜74”杂交的单片段代换系，检测了萌发期和苗期耐寒性的qtl，并建立了qtl金字塔。通过对这些SSSLs及其循环亲本的耐寒性评价，鉴定出萌发期的两个耐寒qtl （qCTBB-5和qCTBB-6）和苗期的两个耐寒qtl （qCTS-6和qCTS-12）。在三个独立的实验中，携带这些qtl的SSSLs比它们的循环亲本HJX74表现出更强的耐寒性。qCTBB-6和qCTS-6定位于同一染色体区域。通过在萌发期和苗期分别携带耐冷QTL的sssl杂交和标记辅助选择（MAS），实现了QTL的金字塔化。选择不同耐寒qtl的金字塔系SC1-1的耐寒性表现出累积效应。研究结果表明，不同的基因（QTLs）控制着水稻萌发期和苗期的耐寒性，通过MAS构建不同发育阶段的耐寒性稳定表达QTLs是预防水稻冷害的良好策略。
Cold stress is one of the main constraints in rice production, and damage from cold can occur at different developmental stages in rice. Understanding the genetic basis of cold tolerance is the key for breeding cold-tolerant variety. In this study, we used single segment substitution lines (SSSLs) derived from a cross between cold-tolerant japonica variety “Nan-yang-zhan” and a popular indica variety “Hua-jing-xian 74” to detect and pyramid QTLs for cold tolerance at the bud bursting and the seedling stages. Evaluation of cold tolerance of these SSSLs and their recurrent parent helped identify two cold-tolerant QTLs (qCTBB-5 and qCTBB-6) at the bud bursting stage and two cold-tolerant QTLs (qCTS-6 and qCTS-12) at the seedling stage. The SSSLs carrying these QTLs showed stronger cold tolerance than their recurrent parent HJX74 did in three independent experiments. The qCTBB-6 and qCTS-6 were mapped to the same chromosomal region. QTL pyramiding was performed by intercrossing of SSSLs carrying the respective QTLs for cold tolerance at the bud bursting stage and the seedling stage and marker-assisted selection (MAS). The selected pyramiding line SC1-1 with different cold-tolerant QTLs showed cumulative effects on cold tolerance. Our results suggest that different genes (QTLs) control cold tolerance at bud bursting and seedling stages, and pyramiding of stable expression QTLs for cold tolerance at different developmental stages through MAS is a good strategy to prevent cold damage in rice.
登录
查看更多内容
影响因子：
5.4
作者：
Suh, J. P.;Jeung, J. U.;Jena, K. K.
通讯作者：
Jena, K. K.
影响因子：
5.8
作者：
Zhihong Zhang;Si-Bin Yu;Ting Yu;Zheng Huang;Ying-guo Zhu
通讯作者：
Zhihong Zhang;Si-Bin Yu;Ting Yu;Zheng Huang;Ying-guo Zhu
影响因子：
1.9
作者：
Shaohong Zhang;Jingsheng Zheng;B. Liu;S. Peng;H. Leung;Junliang Zhao;Xiaofei Wang;Tifeng Yang
通讯作者：
Shaohong Zhang;Jingsheng Zheng;B. Liu;S. Peng;H. Leung;Junliang Zhao;Xiaofei Wang;Tifeng Yang
影响因子：
--
作者：
Shizhong Xu
通讯作者：
Shizhong Xu
DOI：
10.1142/9789812814265_0052
发表时间：
1985
期刊：
--
影响因子：
--
作者：
R. N. Kaw;G. Khush
通讯作者：
R. N. Kaw;G. Khush