The experimental research on the inhibiting impacts of RNAi on Cyclin E in breast cancer cell line
The experimental research on the inhibiting impacts of RNAi on Cyclin E in breast cancer cell line
复制标题
RNAi对乳腺癌细胞Cyclin E抑制作用的实验研究
DOI：
10.1007/s10330-011-0833-4
复制
发表时间：
2011-09
期刊：
影响因子：
--
通讯作者：
Tao Huang
中科院分区：
文献类型：
--
作者：
Wenshan He;Xueqin Li;Na Shen;Tao Huang
目的研究抑制细胞周期蛋白E表达水平对乳腺癌细胞生物学特性的影响。分别在RNA水平和蛋白质水平研究了siRNA对Cyclin E的沉默作用。用CCK-8法检测MCF-7细胞的增殖能力和对化疗药物的敏感性，用流式细胞仪(FCM)检测细胞周期的变化。结果该表达载体可下调细胞周期蛋白E的表达，抑制细胞周期蛋白E的表达，降低细胞的增殖能力，增强细胞对化疗药物的敏感性。结论抑制Cyclin E可抑制乳腺癌细胞的生长，增加其对化疗的敏感性，使其细胞周期减慢。Cyclin E siRNA为进一步研究乳腺癌的基因治疗提供了一个实用的工具。
ObjectiveThe aim of the study was to investigate the biological character change of breast cancer cell line by inhibiting the Cyclin E expressing level.MethodsHuman breast cancer cell line MCF-7 was transfected by Cyclin E siRNA vector pEGFP/CCNE2. SiRNA-induced silencing of Cyclin E was determined at RNA level and protein level, respectively. The proliferation of MCF-7 and its sensitivity to chemical therapy were measured by CCK-8 assay, while cell cycle was measured by flow cytometry (FCM).ResultsThe plasmid could reduce the expression of Cyclin E. The proliferation ability of MCF-7 was decreased and the sensitivity to chemical therapy was enhanced according to the inhibition of Cyclin E expression. The transfected MCF-7 was arrested at G1 phase of cell cycle.ConclusionThe inhibition of Cyclin E can decrease the breast cancer cell’s growth, increase its sensation to chemical therapy and slow down its cell cycle. The Cyclin E siRNA may provide us a practical tool for further study on the gene therapy to breast cancers.
登录
查看更多内容
DOI：
--
发表时间：
2007
期刊：
Journal of Medical Molecular Biology
影响因子：
--
作者：
H. Tao
通讯作者：
H. Tao
DOI：
--
发表时间：
2006-04
期刊：
Analytical and quantitative cytology and histology
影响因子：
--
作者：
A. López-Beltran;G. MacLennan;R. Montironi
通讯作者：
A. López-Beltran;G. MacLennan;R. Montironi
DOI：
10.1023/b:jomg.0000023591.45568.77
发表时间：
2004-01-01
影响因子：
2.5
作者：
Sutherland, RL;Musgrove, EA
通讯作者：
Musgrove, EA
影响因子：
6.4
作者：
Desmedt, Christine;El Ouriaghli, Frank;Sotiriou, Christos
通讯作者：
Sotiriou, Christos
影响因子：
2.5
作者：
Musgrove, Elizabeth A.;Hui, Rina;Sutherland, Robert L.
通讯作者：
Sutherland, Robert L.