Effects of PEO-PPO-PEO triblock copolymers on phospholipid membrane integrity under osmotic stress.
Effects of PEO-PPO-PEO triblock copolymers on phospholipid membrane integrity under osmotic stress.
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DOI：
10.1021/la101841a
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发表时间：
2010-08-03
期刊：
影响因子：
--
通讯作者：
Lee KY
中科院分区：
文献类型：
--
作者：
Wang JY;Chin J;Marks JD;Lee KY
利用巨型单层囊泡（GUVs）研究了PEO-PPO-PEO三嵌段共聚物（主要为poloxam188）在渗透梯度下对磷脂膜完整性的影响。荧光泄漏实验显示，P188对guv结构完整性的两种相反影响取决于其孵育时间的长短。提出了三嵌段共聚物与磷脂膜相互作用的两态过渡机制：吸附态(I)和插入态（II）。而三嵌段共聚物在状态I中起到适度延缓泄漏的作用，它们在状态II中的插入扰乱了脂质堆积，从而增加了膜的通透性。我们的研究结果表明，PEO-PPO-PEO三嵌段共聚物的生物医学应用，无论是作为细胞膜再密封剂还是作为药物递送的促进剂，都是由这两种状态之间的微妙平衡所指导的。
The effects of PEO-PPO-PEO triblock copolymers, mainly Poloxamer 188, on phospholipid membrane integrity under osmotic gradients were explored using giant unilamellar vesicles (GUVs). Fluorescence leakage assays showed two opposing effects of P188 on the structural integrity of GUVs depending on the duration of their incubation time. A two-state transition mechanism of interaction between the triblock copolymers and the phospholipid membrane is proposed: an adsorption (I) and an insertion (II) state. While the triblock copolymer in state I acts to moderately retard the leakage, their insertion in state II perturbs the lipid packing, thus increasing the membrane permeability. Our results suggest that the biomedical application of PEO-PPO-PEO triblock copolymers, either as cell membrane resealing agents or as accelerators for drug delivery, is directed by the delicate balance between these two states.
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