In vivo labeling of adult neural progenitors for MRI with micron sized particles of iron oxide: quantification of labeled cell phenotype.
In vivo labeling of adult neural progenitors for MRI with micron sized particles of iron oxide: quantification of labeled cell phenotype.
复制标题
DOI：
10.1016/j.neuroimage.2008.07.050
复制
发表时间：
2009-02-01
期刊：
影响因子：
5.7
通讯作者：
Koretsky AP
中科院分区：
文献类型：
--
作者：
Sumner JP;Shapiro EM;Maric D;Conroy R;Koretsky AP
室下区（SVZ）是整个成年期神经祖细胞的持续来源。许多旨在研究这些细胞从心室迁移到嗅球或损伤部位等感兴趣部位的动物模型需要组织学来定位前体细胞。在这里，研究证明，在成年啮齿动物中，沿着头端迁移流 (RMS) 迁移的神经祖细胞中，高达 30% 可以通过脑室内注射微米级氧化铁颗粒 (MPIO) 进行标记，用于 MRI。发现从 SVZ 沿着 RMS 迁移的前体细胞在嗅球中填充了所有三种类型的神经细胞；神经元、少突胶质细胞和星形胶质细胞。在所有情况下，10-30% 的细胞在通往嗅球的途中被 RMS 标记。 Ara-C 是一种抗有丝分裂剂，可消除 SVZ、RMS 和嗅球处的前体细胞，并且还消除了前体细胞的 MRI 检测。这表明检测到的 MRI 信号是由于祖细胞离开 SVZ 而不是由于 MPIO 的非特异性扩散。使用 MRI 来可视化个体动物在可塑性或疾病模型期间的神经祖细胞行为应该是一个有用的工具，特别是与 MRI 可以提供的其他信息相结合。
The subventricular zone (SVZ) is a continual source of neural progenitors throughout adulthood. Many of the animal models designed to study the migration of these cells from the ventricle to places of interest like the olfactory bulb or an injury site require histology to localize precursor cells. Here, it is demonstrated that up to 30% of the neural progenitors that migrate along the rostral migratory stream (RMS) in an adult rodent can be labeled for MRI via intraventricular injection of micron sized particles of iron oxide (MPIOs). The precursors migrating from the SVZ along the RMS were found to populate the olfactory bulb with all three types of neural cells; neurons, oligodendrocytes, and astrocytes. In all cases 10-30% of these cells were labeled in the RMS en route to the olfactory bulb. Ara-C an anti-mitotic agent eliminated precursor cells at the SVZ, RMS, and olfactory bulb and also eliminated the MRI detection of the precursors. This indicates that MRI signal detected is due to progenitor cells that leave the SVZ and is not due to non-specific diffusion of MPIOs. Using MRI to visualize neural progenitor cell behavior in individual animals during plasticity or disease models should be a useful tool, especially in combination with other information that MRI can supply.
登录
查看更多内容
影响因子：
16.2
作者：
LUSKIN, MB
通讯作者：
LUSKIN, MB
影响因子：
3.5
作者：
Sumner, James P.;Conroy, Richard;Koretsky, Alan P.
通讯作者：
Koretsky, Alan P.
影响因子：
3.5
作者：
Cayre, M;Bancila, M;Durbec, P
通讯作者：
Durbec, P
DOI：
10.1016/0165-3806(96)00006-5
发表时间：
1996-04-30
期刊：
DEVELOPMENTAL BRAIN RESEARCH
影响因子：
--
作者：
Brunjes, PC;Armstrong, AM
通讯作者：
Armstrong, AM
DOI：
10.1073/pnas.0403918101
发表时间：
2004-07-27
影响因子：
11.1
作者：
Shapiro, EM;Skrtic, S;Koretsky, AP
通讯作者：
Koretsky, AP