A cell-body groove housing the new flagellum tip suggests an adaptation of cellular morphogenesis for parasitism in the bloodstream form of Trypanosoma brucei.
A cell-body groove housing the new flagellum tip suggests an adaptation of cellular morphogenesis for parasitism in the bloodstream form of Trypanosoma brucei.
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DOI：
10.1242/jcs.139139
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发表时间：
2013-12-15
影响因子：
4
通讯作者：
Vaughan S
中科院分区：
文献类型：
--
作者：
Hughes L;Towers K;Starborg T;Gull K;Vaughan S
鞭毛是存在于多种物种中的高度保守的细胞器。在布氏锥虫中，单鞭毛是形态发生、细胞运动和致病所必需的，并且沿着细胞体附着。在细胞分裂周期中，一个新的鞭毛与旧的鞭毛一起形成。在（昆虫）前环形式中，鞭毛连接器（FC）将新鞭毛的尖端连接到旧鞭毛的一侧，确保细胞结构的忠实复制。FC不存在于寄生虫的血流形式中。我们在这里显示，使用新的成像技术，包括连续块面扫描电子显微镜（SBF-SEM），在血流形式的新鞭毛的远端嵌入在细胞体质膜内陷，命名为槽。我们认为，沟有类似的功能鞭毛连接器。沟是一个移动的交界处位于旁边的微管四重奏（MtQ），并发生在一个间隙内的膜下微管紧身衣，导致显着的修改微管在新的鞭毛伸长。这种新的形态发生结构似乎已经进化到可以抵抗哺乳动物血液中的敌对免疫反应。
Flagella are highly conserved organelles present in a wide variety of species. In Trypanosoma brucei the single flagellum is necessary for morphogenesis, cell motility and pathogenesis, and is attached along the cell body. A new flagellum is formed alongside the old during the cell division cycle. In the (insect) procyclic form, the flagella connector (FC) attaches the tip of the new flagellum to the side of the old flagellum, ensuring faithful replication of cell architecture. The FC is not present in the bloodstream form of the parasite. We show here, using new imaging techniques including serial block-face scanning electron microscopy (SBF-SEM), that the distal tip of the new flagellum in the bloodstream form is embedded within an invagination in the cell body plasma membrane, named the groove. We suggest that the groove has a similar function to the flagella connector. The groove is a mobile junction located alongside the microtubule quartet (MtQ) and occurred within a gap in the subpellicular microtubule corset, causing significant modification of microtubules during elongation of the new flagellum. It appears likely that this novel form of morphogenetic structure has evolved to withstand the hostile immune response in the mammalian blood.
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DOI：
10.1098/rstb.1989.0037
发表时间：
1989-06-12
影响因子：
6.3
作者：
SHERWIN, T;GULL, K
通讯作者：
GULL, K
影响因子：
64.5
作者：
Engstler, Markus;Pfohl, Thomas;Overath, Peter
通讯作者：
Overath, Peter
影响因子：
4.8
作者：
Portman, Neil;Lacomble, Sylvain;Gull, Keith
通讯作者：
Gull, Keith
影响因子：
--
作者：
Ralston, KS;Lerner, AG;Hill, KL
通讯作者：
Hill, KL
影响因子：
3
作者：
Hoeoeg, Johanna L.;Bouchet-Marquis, Cedric;Gull, Keith
通讯作者：
Gull, Keith