Effect of dexamethasone on gliosis, ischemia, and dopamine extraction during microdialysis sampling in brain tissue.
Effect of dexamethasone on gliosis, ischemia, and dopamine extraction during microdialysis sampling in brain tissue.
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DOI：
10.1021/ac200782h
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发表时间：
2011-10-15
影响因子：
7.4
通讯作者：
Michael, Adrian C.
中科院分区：
文献类型：
--
作者：
Jaquins-Gerstl, Andrea;Shu, Zhan;Zhang, Jing;Liu, Yansheng;Weber, Stephen G.;Michael, Adrian C.
脑微透析取样是一种分析技术，在神经科学和脑损伤患者的神经重症监护中有许多应用。尽管如此，将微透析探头植入脑组织会导致穿透性损伤，从而引发胶质增生(胶质细胞的激活和增殖)和缺血(血流中断)。因此，探头对受伤的组织进行采样。减轻穿透损伤的影响可能会改进这项技术。合成的糖皮质激素，地塞米松，是一种有效的抗炎和免疫抑制物质。我们在大鼠脑内进行了为期5天的微透析，在灌流液中使用和不使用地塞米松(前24小时10微米，之后2微米)。在灌流的第1天和第4天，我们进行了多巴胺非净流量的测量。第5天取脑组织切片，染色，荧光显微镜下观察。尽管地塞米松能深刻地抑制探针轨迹周围的胶质细胞增生和脑缺血，但它对多巴胺非净通量结果的影响不大。这些发现表明，地塞米松在抑制胶质细胞增生和脑缺血方面非常有效，但其神经保护活性有限。
Microdialysis sampling of the brain is an analytical technique with numerous applications in neuroscience and the neurointensive care of brain-injured human patients. Even so, implanting microdialysis probes into brain tissue causes a penetration injury that triggers gliosis (the activation and proliferation of glial cells) and ischemia (the interruption of blood flow). Thus, the probe samples injured tissue. Mitigating the effects of the penetration injury might refine the technique. The synthetic glucocorticoid, dexamethasone, is a potent anti-inflammatory and immunosuppressant substance. We performed microdialysis in the rat brain for 5 days, with and without dexamethasone in the perfusion fluid (10 µM for the first 24 hrs and 2 µM thereafter). On the 1st and 4th day of the perfusion, we performed dopamine no-net-flux measurements. On the 5th day, we sectioned and stained the brain tissue and examined it by fluorescence microscopy. Although dexamethasone profoundly inhibited gliosis and ischemia around the probe tracks it had only modest effects on dopamine no-net-flux results. These findings show that dexamethasone is highly effective at suppressing gliosis and ischemia but is limited in its neuroprotective activity.
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