Image-guided pro-angiogenic therapy in diabetic stroke mouse models using a multi-modal nanoprobe.
Image-guided pro-angiogenic therapy in diabetic stroke mouse models using a multi-modal nanoprobe.
复制标题
使用多模态纳米探针对糖尿病中风小鼠模型进行图像引导促血管生成治疗
作者：
Bai YY;Gao X;Wang YC;Peng XG;Chang D;Zheng S;Li C;Ju S
目的：用目前的诊断方法很难评估促血管生成治疗的疗效。目的是开发一种非侵入性的成像策略，以确定血管生成的时间特征，并评估糖尿病卒中小鼠模型对促血管生成治疗的反应。方法：将自制的ανβ-3整合素靶向多模式纳米探针静脉注射到光栓性卒中小鼠模型中。注射后24 h行磁共振成像(MRI)和近红外荧光成像(NIRF)检查。将骨髓来源的血管内皮祖细胞(EPC)注入缺血性卒中小鼠模型中，以刺激血管生成。结果：糖尿病组和野生型小鼠脑缺血-血管形成区信号强度在第10天达到峰值，糖尿病模型组信号增强程度明显低于正常对照组。尽管在EPC治疗后，两种模型的信号强度都显著升高，但与野生型对照组相比，糖尿病动物的信号增强不那么明显。组织学分析显示，β-3整合素的表达和微血管密度与核磁共振和激光核磁共振成像的信号强度相关。结论：无创成像方法可用于早期、准确地评估糖尿病卒中模型中促血管生成治疗的反应。
Purpose: The efficacy of pro-angiogenic therapy is difficult to evaluate with current diagnostic modalities. The objectives were to develop a non-invasive imaging strategy to define the temporal characteristics of angiogenesis and to evaluate the response to pro-angiogenic therapy in diabetic stroke mouse models. Methods: A home-made ανβ3 integrin-targeted multi-modal nanoprobe was intravenously injected into mouse models at set time points after photothrombotic stroke. Magnetic resonance imaging (MRI) and near-infrared fluorescence (NIRF) imaging were carried out at 24 h post-injection. Bone marrow-derived endothelial progenitor cells (EPCs) were infused into the mouse models of ischemic stroke to stimulate angiogenesis. Results: The peak signal intensity in the ischemic-angiogenic area of diabetic and wild-type mouse models was achieved on day 10, with significantly lower signal enhancement observed in the diabetic models. Although the signal intensity was significantly higher after EPC treatment in both models, the enhancement was less pronounced in the diabetic animals compared with the wild-type controls. Histological analysis revealed that the microvessel density and expression of β3 integrin were correlated with the signal intensity assessed with MRI and NIRF imaging. Conclusions: The non-invasive imaging method could be used for early and accurate evaluation of the response to pro-angiogenic therapy in diabetic stroke models.
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影响因子：
56.9
作者：
Asahara, T;Murohara, T;Isner, JM
通讯作者：
Isner, JM
影响因子：
7.7
作者：
Navaratna D;Guo SZ;Hayakawa K;Wang X;Gerhardinger C;Lo EH
通讯作者：
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影响因子：
8.3
作者：
Li Q;Atochin D;Kashiwagi S;Earle J;Wang A;Mandeville E;Hayakawa K;d'Uscio LV;Lo EH;Katusic Z;Sessa W;Huang PL
通讯作者：
Huang PL
影响因子：
8.3
作者：
Klohs, Jan;Graefe, Michael;Wunder, Andreas
通讯作者：
Wunder, Andreas
影响因子：
8.3
作者：
KRUPINSKI, J;KALUZA, J;WANG, JM
通讯作者：
WANG, JM