Bacterial diversity in Bercaea Cruentata gut described using high-throughput sequencing
Bacterial diversity in Bercaea Cruentata gut described using high-throughput sequencing
复制标题
使用高通量测序描述 Bercaea Cruentata 肠道中的细菌多样性
DOI：
10.1016/j.fsigss.2015.09.190
复制
发表时间：
2015-12
影响因子：
--
通讯作者：
Y.D. Guo
中科院分区：
文献类型：
--
作者：
L.L. Long;J.J. Guo;P. Li;Y.D. Guo
死后间隙调查是法医检验的主要和重要环节。法医昆虫学是科学估计死亡时间的有用工具。由于细菌在吸引昆虫到尸体上起着至关重要的作用，因此了解细菌和昆虫之间的相互作用对法医科学家来说至关重要。本研究的目的是研究白藓菌（bercaea cruentata）肠道细菌多样性。收集成虫和三龄幼虫的肠道菌群。cruentata。采用DNA技术（在Illumina MiSeq平台上高通量测序16S rRNA扩增）对细菌群落进行编目。成虫组的操作分类单位（OTUs）丰度较高，幼虫和成虫组均以厚壁菌门和变形菌门为主。我们的研究提供了肠道细菌多样性的数据。这是第一次。成人细菌群落。Cruentatagut与幼虫肠道的分类丰富度和相对丰度存在差异，但成虫和幼虫对特定的细菌种类都有偏好。食糜昆虫吸引细菌的信号机制有待进一步研究。
The investigation of postmortem interval (PMI) is the main and important course for forensic examination. Forensic entomology is a useful tool to estimate scientifically the time elapsed since death. Since bacteria play a critical role in attracting insects to cadavers, it is essential for forensic scientists to understand the interactivation between bacteria and insects. This study aimed to investigate gut bacteria diversity ofBercaea cruentata. The gut microflora was collected from adult and third instars larvae ofB. cruentata.DNA technologies was used (high-throughput sequencing of 16S rRNA amplification on Illumina MiSeq platform) to catalog the bacterial community. Operational taxonomic units (OTUs) were of higher abundance in adult group, and Firmicutes andProteobacteriawere both predominated in larvae and adult groups. Our study provided data on gut bacteria diversity ofB. cruentatafor the first time. Bacterial community in adultB. cruentatagut were observed different taxon richness and relative abundance patterns compared with the larvae gut, but both adult and larvae have preferences for specific bacteria species. Further studies are required to verify the signaling mechanism for bacterial attraction of sarcosaphagous insects.
登录
查看更多内容
影响因子：
2.2
作者：
L. E. Visôtto;M. G. A. Oliveira;R. Guedes;A. Ribon;P. I. V. Good-God
通讯作者：
L. E. Visôtto;M. G. A. Oliveira;R. Guedes;A. Ribon;P. I. V. Good-God
DOI：
10.1007/978-1-4020-9684-6_11
发表时间：
2009
期刊：
--
影响因子：
--
作者：
H. LeBlanc;J. Logan
通讯作者：
H. LeBlanc;J. Logan
DOI：
10.1073/pnas.0604865103
发表时间：
2006-10-10
影响因子：
11.1
作者：
Broderick, Nichole A.;Raffa, Kenneth F.;Handelsman, Jo
通讯作者：
Handelsman, Jo
红金金蝇（Macquart）（双翅目：丽蝇科）蛹形态发生的研究，用于死后间隔估计
影响因子：
2.2
作者：
Ma, Ting;Huang, Jia;Wang, Jiang-Feng
通讯作者：
Wang, Jiang-Feng