Neurite Outgrowth in PC12 Cells Stimulated by Components from Dendranthema × grandiflorum cv. "Mottenohoka" Is Enhanced by Suppressing Phosphorylation of p38MAPK.
Neurite Outgrowth in PC12 Cells Stimulated by Components from Dendranthema × grandiflorum cv. "Mottenohoka" Is Enhanced by Suppressing Phosphorylation of p38MAPK.
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DOI：
10.1155/2013/403503
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发表时间：
2013
期刊：
影响因子：
--
通讯作者：
Furukawa S
中科院分区：
文献类型：
--
作者：
Nishina A;Kimura H;Tsukagoshi H;Kozawa K;Koketsu M;Ninomiya M;Furukawa S
桔梗菊（dendr菊× grandflorum）的成分。鉴定了促进PC12细胞神经突生长的“Mottenohoka”，并研究了分离成分刺激神经突生长的机制。分离出促进PC12细胞胞外信号调节激酶1/2 （ERK 1/2）磷酸化的组分。通过结构分析，鉴定其有效成分为木犀草素和木犀草素。电印迹法和免疫染色法观察阿卡辛或木犀草素对PC12细胞的影响。木犀草素或阿卡乙酰素刺激PC12细胞培养2天后，观察到有轻微的神经突生长。然而，相比之下，神经生长因子刺激诱导了显著的神经突生长。用SB203580（一种p38MAPK抑制剂）预处理木犀草素或阿卡乙酰素可显著促进神经突生长。本研究通过黄酮类化合物磷酸化ERK 1/2和p38MAPK的结果表明，抑制p38MAPK的磷酸化可以有效地促进神经突的生长。
Components from Dendranthema × grandiflorum cv. “Mottenohoka” that promote neurite outgrowth of PC12 cells were identified and the mechanism of neurite outgrowth stimulated by isolated components was studied. Components that promoted the phosphorylation of extracellular signal-regulated kinase 1/2 (ERK 1/2) of PC12 cells were isolated. From various structural analyses, the active components were identified as acacetin and luteolin. The effects of acacetin or luteolin on PC12 cells were evaluated by electro-blotting and immunostaining. Slight neurite outgrowth in PC12 cells was observed within 2 days of culture after stimulation by luteolin or acacetin. However, NGF-stimulation induced remarkable neurite outgrowth in comparison. Neurite outgrowth by luteolin or acacetin was significantly enhanced by pretreatment with SB203580 (a p38MAPK inhibitor). The results of this study into the phosphorylation of ERK 1/2 and p38MAPK by flavonoids suggest that the inhibition of p38MAPK phosphorylation may effectively enhance neurite outgrowth.
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