Anterior and posterior corneal stroma elasticity assessed using nanoindentation.
Anterior and posterior corneal stroma elasticity assessed using nanoindentation.
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DOI：
10.1016/j.exer.2013.06.004
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发表时间：
2013-10
影响因子：
3.4
通讯作者：
Ziebarth, Noel M.
中科院分区：
文献类型：
--
作者：
Dias, Janice M.;Ziebarth, Noel M.
角膜生物力学是发展角膜相关疾病的诊断和治疗方法的重要参数。广泛认为角膜的机械强度来源于基质的胶原成分。然而，需要更全面地了解基质内的机械特性，以改善当前的角膜诊断和治疗技术。本研究的目的是利用原子力显微镜（AFM）对人角膜前、后基质区进行弹性表征。将9对人全眼球置于20%葡聚糖溶液中，角膜面朝下，以将角膜厚度恢复至生理水平（400-600μm）。从所有眼中去除上皮和Bowman膜。然后对左眼（OS）进行前基质AFM弹性测试。从右眼（OD）去除额外的基质，以允许在原始厚度的50%的深度处测量后基质。所有实验均使用浸没在15%葡聚糖中的角膜进行，以保持角膜水合。研究结果显示，前基质的杨氏弹性模量（平均：281 ± 214 kPa;范围：59- 764 kPa）显著高于后基质的杨氏弹性模量（平均：89.5 ± 46.1kPa;范围：29- 179 kPa）（p=0.014）。此外，后基质弹性和前基质弹性之间存在线性关系（p=0.0428）。平均而言，后基质的弹性是前基质的39.3%。总之，角膜基质内似乎存在弹性梯度，在设计和开发角膜诊断和治疗方法时应考虑到这一点，以提高疗效。
Corneal biomechanics is an essential parameter for developing diagnostic and treatment methods of corneal-related diseases. It is widely accepted that corneal mechanical strength stems from the stroma's collagenous composition. However, more comprehensive insight into the mechanical properties within the stroma is needed to improve current corneal diagnostic and treatment techniques. The purpose of this study was to perform elasticity characterization of anterior and posterior stromal regions of human corneas using atomic force microscopy (AFM). Nine pairs of human whole globes were placed in 20% Dextran solution, cornea side down, to restore the corneal thickness to physiological levels (400-600μm). The epithelium and Bowman's membrane were removed from all eyes. Anterior stromal AFM elasticity testing was then performed on left (OS) eyes. Additional stroma was removed from right (OD) eyes to allow posterior stromal measurements at a depth of 50% of the original thickness. All experiments were performed with corneas submerged in 15% Dextran to maintain corneal hydration. The results of the study showed that the Young's modulus of elasticity of the anterior stroma (average: 281 ± 214kPa; range: 59-764kPa) was significantly higher than that of the posterior stroma (average: 89.5 ± 46.1kPa; range: 29-179kPa) (p=0.014). In addition, a linear relationship was found between the posterior stromal elasticity and anterior stromal elasticity (p=0.0428). On average, the elasticity of the posterior stroma is 39.3% of the anterior stroma. In summary, there appears to be an elasticity gradient within the corneal stroma, which should be considered in the design and development of corneal diagnostic and treatment methods to enhance efficacy.
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