In vitro differentiation of human embryonic stem cells to hemogenic endothelium and blood progenitors via embryoid body formation.
In vitro differentiation of human embryonic stem cells to hemogenic endothelium and blood progenitors via embryoid body formation.
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DOI：
10.1016/j.xpro.2021.100367
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发表时间：
2021-03-19
期刊：
影响因子：
--
通讯作者：
Kouskoff V
中科院分区：
文献类型：
--
作者：
Garcia-Alegria E;Potts B;Menegatti S;Kouskoff V
由于伦理原因和胚胎获取受到限制，人们对人类胚胎发生过程中血液祖细胞的出现知之甚少。使用人类胚胎干细胞 (hESC) 作为模型系统为剖析人类血细胞的形成提供了独特的机会。在这里，我们描述了一个协议，允许 hESC 通过拟胚体分化为造血内皮细胞，并随后分化为血液祖细胞。该方案依赖于拟胚体的形成，如果不仔细执行，这会很棘手。有关该协议的使用和执行的完整详细信息，请参阅。 hESC 向血细胞祖细胞的分化 通过类胚体形成的分化方案 从第 6 天的类胚体中分离造血内皮 测定造血内皮造血输出的测定 由于伦理原因和胚胎获取受限，人们对人类胚胎发生过程中造血祖细胞的出现知之甚少。使用人类胚胎干细胞 (hESC) 作为模型系统为剖析人类血细胞的形成提供了独特的机会。在这里，我们描述了一个协议，允许 hESC 通过拟胚体分化为造血内皮细胞，并随后分化为血液祖细胞。该方案依赖于拟胚体的形成，如果不仔细执行，这会很棘手。
Little is known about the emergence of blood progenitors during human embryogenesis due to ethical reasons and restricted embryo access. The use of human embryonic stem cells (hESCs) as a model system offers unique opportunities to dissect human blood cell formation. Here, we describe a protocol allowing the differentiation of hESCs via embryoid bodies toward hemogenic endothelium and its subsequent differentiation to blood progenitors. This protocol relies on the formation of embryoid bodies, which is tricky if not carefully performed. For complete details on the use and execution of this protocol, please refer to. Differentiation of hESCs to blood cell progenitors Differentiation protocol via embryoid body formation Isolation of hemogenic endothelium from day 6 embryoid bodies Assays to determine hemogenic endothelium hematopoietic output Little is known about the emergence of blood progenitors during human embryogenesis due to ethical reasons and restricted embryo access. The use of human embryonic stem cells (hESCs) as a model system offers unique opportunities to dissect human blood cell formation. Here, we describe a protocol allowing the differentiation of hESCs via embryoid bodies toward hemogenic endothelium and its subsequent differentiation to blood progenitors. This protocol relies on the formation of embryoid bodies, which is tricky if not carefully performed.
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影响因子：
5.9
作者：
Garcia-Alegria E;Menegatti S;Fadlullah MZH;Menendez P;Lacaud G;Kouskoff V
通讯作者：
Kouskoff V
影响因子：
8.8
作者：
Park MA;Kumar A;Jung HS;Uenishi G;Moskvin OV;Thomson JA;Slukvin II
通讯作者：
Slukvin II
影响因子：
30.5
作者：
Schmitt, TM;de Pooter, RF;Zúñiga-Pflücker, JC
通讯作者：
Zúñiga-Pflücker, JC
影响因子：
3.7
作者：
Niwa A;Heike T;Umeda K;Oshima K;Kato I;Sakai H;Suemori H;Nakahata T;Saito MK
通讯作者：
Saito MK
影响因子：
5.9
作者：
Tursky, Melinda L.;Loi, To Ha;Molloy, Timothy J.
通讯作者：
Molloy, Timothy J.