Arabidopsis fatty acid desaturase FAD2 is required for salt tolerance during seed germination and early seedling growth.
Arabidopsis fatty acid desaturase FAD2 is required for salt tolerance during seed germination and early seedling growth.
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拟南芥脂肪酸去饱和酶 FAD2 是种子萌发和早期幼苗生长过程中耐盐性所必需的
DOI：
10.1371/journal.pone.0030355
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发表时间：
2012
期刊：
影响因子：
3.7
通讯作者：
Zhang H
中科院分区：
文献类型：
--
作者：
Zhang J;Liu H;Sun J;Li B;Zhu Q;Chen S;Zhang H
脂肪酸去饱和酶在植物对非生物胁迫的响应中起着重要作用。然而，它们在植物抗盐胁迫中的确切功能尚不清楚。在这项工作中，我们提供的证据表明，FAD 2，内质网定位的ω-6去饱和酶，是拟南芥耐盐性所必需的。使用空泡和质膜囊泡制备的野生型（Col-0）和功能丧失的拟南芥突变体，fad 2，它缺乏功能FAD 2的叶子，我们研究了脂肪酸组成和Na+依赖H+运动的孤立的囊泡。我们观察到，当与Col-0相比，液泡和质膜多不饱和度的水平较低，Na+/H+交换活性降低液泡和质膜小泡分离fad 2突变体。CoroNa-Green染色、Na+净流出量和耐盐性分析表明，fad 2在种子萌发和幼苗生长早期，根细胞胞质中积累较多的Na+，对盐胁迫更为敏感，这与Na+/H+交换活性降低相一致。我们的研究结果表明，FAD 2介导的高水平的液泡和质膜脂肪酸去饱和是必要的膜附着的Na+/H+交换器的正常功能，从而保持低的细胞溶质Na+浓度的耐盐性在种子萌发和早期幼苗生长在拟南芥。
Fatty acid desaturases play important role in plant responses to abiotic stresses. However, their exact function in plant resistance to salt stress is unknown. In this work, we provide the evidence that FAD2, an endoplasmic reticulum localized ω-6 desaturase, is required for salt tolerance in Arabidopsis. Using vacuolar and plasma membrane vesicles prepared from the leaves of wild-type (Col-0) and the loss-of-function Arabidopsis mutant, fad2, which lacks the functional FAD2, we examined the fatty acid composition and Na+-dependent H+ movements of the isolated vesicles. We observed that, when compared to Col-0, the level of vacuolar and plasma membrane polyunsaturation was lower, and the Na+/H+ exchange activity was reduced in vacuolar and plasma membrane vesicles isolated from fad2 mutant. Consistent with the reduced Na+/H+ exchange activity, fad2 accumulated more Na+ in the cytoplasm of root cells, and was more sensitive to salt stress during seed germination and early seedling growth, as indicated by CoroNa-Green staining, net Na+ efflux and salt tolerance analyses. Our results suggest that FAD2 mediated high-level vacuolar and plasma membrane fatty acid desaturation is essential for the proper function of membrane attached Na+/H+ exchangers, and thereby to maintain a low cytosolic Na+ concentration for salt tolerance during seed germination and early seedling growth in Arabidopsis.
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