Tissue factor activity of small and large extracellular vesicles in different diseases.
Tissue factor activity of small and large extracellular vesicles in different diseases.
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不同疾病中细胞外小泡和大泡的组织因子活性。
DOI：
10.1016/j.rpth.2023.100124
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发表时间：
2023-03
影响因子：
4.6
通讯作者：
Mackman, Nigel
中科院分区：
文献类型：
--
作者：
Sachetto, Ana T. A.;Archibald, Sierra J.;Hisada, Yohei;Rosell, Axel;Havervall, Sebastian;van Es, Nick;Nieuwland, Rienk;Campbell, Robert A.;Middleton, Elizabeth A.;Rondina, Matthew T.;Thalin, Charlotte;Mackman, Nigel
循环促凝血细胞外囊泡 (EV) 在癌症、败血症和 COVID-19 等疾病中增加。 EV 组织因子 (TF) 活性与胰腺癌和 COVID-19 患者脓毒症和静脉血栓形成中的弥散性血管内凝血相关。 EV 通常通过〜20,000 g 离心分离。在这项研究中，我们分析了脓毒症、胰腺癌或 COVID-19 患者中富含大和小 EV 的 2 个 EV 群体的 TF 活性。通过在 20,000 g（大型 EV、LEV）和 100,000 g（小型 EV、SEV）下连续离心，从血浆中分离出 EV。我们分析了来自有或没有脂多糖 (LPS) 刺激的健康个体的全血样本制备的血浆中的 EV，以及来自脓毒症、胰腺癌或 COVID-19 患者血浆样本中的 EV。测量了 EV 的 TF 依赖性（EV-TF 活性）和 TF 独立因子 Xa (FXa) 生成。 LPS 增加了 LEV 中的 EV-TF 活性，但不增加 SEV 中的 EV-TF 活性。同样，在 2 名 EV-TF 活性高于检测背景的脓毒症患者中，我们观察到 LEV 中的 EV-TF 活性，而不是 SEV 中的 EV-TF 活性。胰腺癌或 COVID-19 患者的 LEV 和 SEV 中均存在循环 EV-TF 活性。我们建议通过以 100,000 g（而不是 20,000 g）离心的方式从患者血浆中分离出 EV，以便更准确地测量循环 EV-TF 活性水平。循环细胞外囊泡（EV）-组织因子（TF）活性与不同疾病中的血栓形成相关。我们测量了不同疾病中小型（20K）和大型（100K）EV 的 TF 活性。胰腺癌和 COVID-19 患者的小型和大型 EV 中均存在 TF 活性。我们建议通过 100,000 g 离心测量从血浆中分离出的 EV 的 TF 活性。
Circulating procoagulant extracellular vesicles (EVs) are increased in diseases, such as cancer, sepsis, and COVID-19. EV tissue factor (TF) activity is associated with disseminated intravascular coagulation in sepsis and venous thrombosis in patients with pancreatic cancer and COVID-19. EVs are commonly isolated by centrifugation at ∼20,000 g. In this study, we analyzed the TF activity of 2 EV populations enriched for large and small EVs in patients with either sepsis, pancreatic cancer, or COVID-19. EVs were isolated from plasma by sequential centrifugation at 20,000 g (large EVs, LEVs) and then 100,000 g (small EVs, SEVs). We analyzed EVs from plasma prepared from whole blood samples from healthy individuals with or without lipopolysaccharide (LPS) stimulation as well as EVs from plasma samples from patients with either sepsis, pancreatic cancer, or COVID-19. TF-dependent (EV-TF activity) and TF-independent factor Xa (FXa) generation of the EVs was measured. LPS increased EV-TF activity in LEVs but not SEVs. Similarly, in 2 patients with sepsis who had EV-TF activity above the background of the assay we observed EV-TF activity in LEVs but not SEVs. Patients with pancreatic cancer or COVID-19 had circulating EV-TF activity in both LEVs and SEVs. We recommend that EVs are isolated from plasma from patients by centrifugation at 100,000 g rather than 20,000 g to obtain a more accurate measure of levels of circulating EV-TF activity. Circulating extracellular vesicles (EV)-tissue factor (TF) activity is associated with thrombosis in different diseases. We measured TF activity of small (20K) and large (100K) EVs in different diseases. TF activity was present in small and large EVs in patients with pancreatic cancer and COVID-19. We recommend measuring TF activity of EVs isolated from plasma by centrifugation at 100,000 g.
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影响因子：
16
作者：
Krishnamachary B;Cook C;Kumar A;Spikes L;Chalise P;Dhillon NK
通讯作者：
Dhillon NK
影响因子：
1.3
作者：
Avenick, Daniel;Kidd, Linda;Mackman, Nigel
通讯作者：
Mackman, Nigel
影响因子：
5.7
作者：
Key, Nigel S.;Mackman, Nigel
通讯作者：
Mackman, Nigel
影响因子：
4.6
作者：
Kasthuri, Raj S.;Hisada, Yohei;Mackman, Nigel
通讯作者：
Mackman, Nigel
DOI：
10.1111/j.1538-7836.2011.04488.x
发表时间：
2011-11
期刊：
Journal of thrombosis and haemostasis : JTH
影响因子：
--
作者：
Aleman MM;Gardiner C;Harrison P;Wolberg AS
通讯作者：
Wolberg AS