Transcriptome and Physiological Analyses of a Navel Orange Mutant with Improved Drought Tolerance and Water Use Efficiency Caused by Increases of Cuticular Wax Accumulation and ROS Scavenging Capacity.
Transcriptome and Physiological Analyses of a Navel Orange Mutant with Improved Drought Tolerance and Water Use Efficiency Caused by Increases of Cuticular Wax Accumulation and ROS Scavenging Capacity.
复制标题
DOI：
10.3390/ijms23105660
复制
发表时间：
2022-05-18
影响因子：
5.6
通讯作者：
中科院分区：
文献类型：
--
作者：
干旱是限制柑橘品质和产量的主要非生物胁迫之一。角质层蜡质在调节植物耐旱性和水分利用效率方面具有重要作用。然而，角质层蜡质对柑橘耐旱性、水分利用效率的影响及其分子机制尚不清楚。'龙辉红'（MT）是纽荷尔脐橙橙子的芽变品种，叶片卷曲、鲜艳。在本研究中，MT叶片中总蜡和脂肪族蜡化合物，包括正烷烃，正伯醇和正醛的量显著增加，导致角质层渗透性降低，最终导致耐旱性和WUE的提高。干旱胁迫下，MT叶片中丙二醛（MDA）和过氧化氢（H2O2）含量显著低于WT，脯氨酸和可溶性糖含量显著高于WT，超氧化物歧化酶（SOD）、过氧化氢酶（CAT）和过氧化物酶（POD）活性显著高于WT，这可能与MT减轻了活性氧（ROS）的伤害，提高了渗透调节能力和细胞膜稳定性有关。提高MT对干旱胁迫的耐受性。转录组测序结果表明，7个结构基因参与了蜡质的合成与输出、MAPK级联反应和ROS清除，其中7个转录因子编码基因可能在促进MT植物表皮蜡质积累、提高耐旱性和WUE中发挥重要作用。这些结果不仅证实了角质层蜡质在调节柑橘抗旱性和水分利用效率中的重要作用，而且为提高柑橘抗旱性和水分利用效率提供了多种候选基因。
Drought is one of the main abiotic stresses limiting the quality and yield of citrus. Cuticular waxes play an important role in regulating plant drought tolerance and water use efficiency (WUE). However, the contribution of cuticular waxes to drought tolerance, WUE and the underlying molecular mechanism is still largely unknown in citrus. ‘Longhuihong’ (MT) is a bud mutant of ‘Newhall’ navel orange with curly and bright leaves. In this study, significant increases in the amounts of total waxes and aliphatic wax compounds, including n-alkanes, n-primary alcohols and n-aldehydes, were overserved in MT leaves, which led to the decrease in cuticular permeability and finally resulted in the improvements in drought tolerance and WUE. Compared to WT leaves, MT leaves possessed much lower contents of malondialdehyde (MDA) and hydrogen peroxide (H2O2), significantly higher levels of proline and soluble sugar, and enhanced superoxide dismutase (SOD), catalase (CAT) and peroxidase (POD) activities under drought stress, which might reduce reactive oxygen species (ROS) damage, improve osmotic regulation and cell membrane stability, and finally, enhance MT tolerance to drought stress. Transcriptome sequencing results showed that seven structural genes were involved in wax biosynthesis and export, MAPK cascade, and ROS scavenging, and seven genes encoding transcription factors might play an important role in promoting cuticular wax accumulation, improving drought tolerance and WUE in MT plants. Our results not only confirmed the important role of cuticular waxes in regulating citrus drought resistance and WUE but also provided various candidate genes for improving citrus drought tolerance and WUE.
登录
查看更多内容
在干旱胁迫下，fragaria vesca中AP2/EREBP的全基因组鉴定以及FVDREB亚科的表达模式分析。
影响因子：
5.3
作者：
Dong C;Xi Y;Chen X;Cheng ZM
通讯作者：
Cheng ZM
影响因子：
11.6
作者：
Go, Young Sam;Kim, Hyojin;Suh, Mi Chung
通讯作者：
Suh, Mi Chung
影响因子：
5.2
作者：
Gago, Jorge;Douthe, Cyril;Medrano, Hipolitd
通讯作者：
Medrano, Hipolitd
黄莎草 WRI4 样基因通过促进角质层蜡质生物合成提高拟南芥的耐旱性
影响因子：
5.3
作者：
Cheng C;Hu S;Han Y;Xia D;Huang BL;Wu W;Hussain J;Zhang X;Huang B
通讯作者：
Huang B
「冰糖」甜橙果实贮藏过程中角质层组成及晶体结构的变化
影响因子：
2.9
作者：
Ding, Shenghua;Zhang, Ding;Shan, Yang
通讯作者：
Shan, Yang