Yellow nutsedge WRI4-like gene improves drought tolerance in Arabidopsis thaliana by promoting cuticular wax biosynthesis.
Yellow nutsedge WRI4-like gene improves drought tolerance in Arabidopsis thaliana by promoting cuticular wax biosynthesis.
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黄莎草 WRI4 样基因通过促进角质层蜡质生物合成提高拟南芥的耐旱性
DOI：
10.1186/s12870-020-02707-7
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发表时间：
2020-10-31
影响因子：
5.3
通讯作者：
Huang B
中科院分区：
文献类型：
--
作者：
Cheng C;Hu S;Han Y;Xia D;Huang BL;Wu W;Hussain J;Zhang X;Huang B
背景角质层蜡质在保护植物免受干旱胁迫中起着重要作用。油莎草（Cyperus esculentus）（黄莎草）是一种生长在热带和温带以及较冷地区的坚韧杂草。本研究报道了油莎草WRI 4-like基因的分子克隆及其在拟南芥中的功能研究。结果利用RACE PCR，从黄莎草中扩增出全长WRI-like基因。系统发育分析和氨基酸序列比较表明，该基因为一个WRI 4类基因。根据组织特异性表达数据，WRI 4-like基因在叶片中的表达量最高，其次是根和块茎。转nutsedgeWRI 4-like基因的拟南芥表现出更强的耐旱性。结果表明，转基因株系的气孔导度、蒸腾速率、叶绿素淋失、水分损失显著降低，水分利用效率（WUE）显著提高。在无干旱胁迫下，转基因株系与野生型之间脂肪酸合成关键基因的表达量无显著差异，而表皮蜡合成相关基因的表达量显著高于野生型。PEG模拟的干旱胁迫显著增加了转基因拟南芥品系中脂肪酸和蜡质生物合成关键基因的表达，但在WT植物中没有。结论油莎豆WRI 4-like基因可能通过促进表皮蜡质的合成和沉积，提高拟南芥的耐旱性。这反过来又降低了叶绿素淋失、气孔导度、蒸腾速率、水分损失，并提高了干旱胁迫条件下的水分利用效率。因此，CeWRI 4-like基因有望成为提高作物耐旱性的候选基因。
BackgroundCuticular wax plays important role in protecting plants from drought stress. In ArabidopsisWRI4improves drought tolerance by regulating the biosynthesis of fatty acids and cuticular wax.Cyperus esculentus(yellow nutsedge) is a tough weed found in tropical and temperate zones as well as in cooler regions. In the current study, we report the molecular cloning of aWRI4-like gene fromCyperus esculentusand its functional characterization in Arabidopsis.ResultsUsing RACE PCR, full-lengthWRI-like gene was amplified from yellow nutsedge. Phylogenetic analyses and amino acid comparison suggested it to be aWRI4-like gene. According to the tissue-specific expression data, the highest expression ofWRI4-like gene was found in leaves, followed by roots and tuber. Transgenic Arabidopsis plants expressing nutsedgeWRI4-like gene manifested improved drought stress tolerance. Transgenic lines showed significantly reduced stomatal conductance, transpiration rate, chlorophyll leaching, water loss and improved water use efficiency (WUE). In the absence of drought stress, expression of key genes for fatty acid biosynthesis was not significantly different between transgenic lines and WT while that of cuticular wax biosynthesis genes was significantly higher in transgenic lines than WT. The PEG-simulated drought stress significantly increased expression of key genes for fatty acid as well as wax biosynthesis in transgenic Arabidopsis lines but not in WT plants. Consistent with the gene expression data, cuticular wax load and deposition was significantly higher in stem and leaves of transgenic lines compared with WT under control as well as drought stress conditions.ConclusionsWRI4-like gene fromCyperus esculentusimproves drought tolerance in Arabidopsis probably by promoting cuticular wax biosynthesis and deposition. This in turn lowers chlorophyll leaching, stomatal conductance, transpiration rate, water loss and improves water use efficiency under drought stress conditions. Therefore,CeWRI4-like gene could be a good candidate for improving drought tolerance in crops.
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影响因子：
9.4
作者：
Franks, Peter J.;Doheny-Adams, Timothy W.;Gray, Julie E.
通讯作者：
Gray, Julie E.
影响因子：
4.8
作者：
GINDEL, I
通讯作者：
GINDEL, I
影响因子：
7.2
作者：
Cernac, A;Benning, C
通讯作者：
Benning, C
影响因子：
2.6
作者：
Bado S;Bazongo P;Son G;Kyaw MT;Forster BP;Nielen S;Lykke AM;Ouédraogo A;Bassolé IH
通讯作者：
Bassolé IH
影响因子：
4.1
作者：
FLOOD, AE;PRIESTLE.CA
通讯作者：
PRIESTLE.CA