Measurement of viscoelastic properties in multiple anatomical regions of acute rat brain tissue slices.
Measurement of viscoelastic properties in multiple anatomical regions of acute rat brain tissue slices.
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DOI：
10.1016/j.jmbbm.2013.08.026
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发表时间：
2014-01
影响因子：
3.9
通讯作者：
Sarntinoranont M
中科院分区：
文献类型：
--
作者：
Lee SJ;King MA;Sun J;Xie HK;Subhash G;Sarntinoranont M
脑组织的力学性质数据是理解神经疾病的生物力学和大脑对不同机械和外科力量的反应所必需的。大多数研究都描述了脑组织在大范围内的力学行为，或者只对灰质或白质区域的组织属性进行了分类。在这项研究中，使用光学相干断层扫描(OCT)压痕系统测量了不同解剖区域包括大脑皮层、尾状核/壳核和海马区的大鼠脑组织切片的空间非均匀粘弹性特性。细胞存活率也被测试，以观察神经元变性和组织切片的形态变化，并为力学测试提供适当的时间线。通过将归一化变形数据(D/ti)(定义为变形深度(D)与组织切片初始厚度(Ti)的比率)拟合到粘弹性有限元模型来估计剪切模量。大脑皮层、海马区和尾壳核的瞬时到平衡剪切模量值的范围分别为3.8~0.54kPa、1.4~0.27kPa和1.0~0.17kPa。虽然这些区域都是灰质结构，但它们测量的力学性能明显不同。准确测量区域间力学特性的差异将有助于更好地了解器官水平的结构参数和中枢神经系统组织内对变形损伤的区域差异易感性。
Mechanical property data for brain tissue are needed to understand the biomechanics of neurological disorders and response of the brain to different mechanical and surgical forces. Most studies have characterized mechanical behavior of brain tissues over large regions or classified tissue properties for either gray or white matter regions only. In this study, spatially heterogeneous viscoelastic properties of ex vivo rat brain tissue slices were measured in different anatomical regions including the cerebral cortex, caudate/putamen, and hippocampus using an optical coherence tomography (OCT) indentation system. Cell viability was also tested to observe neuronal degeneration and morphological changes in tissue slices and provide a proper timeline for mechanical tests. Shear modulus was estimated by fitting normalized deformation data (D/ti), which was defined as the ratio of deformation depth (D) to initial thickness of the tissue slice (ti), to a viscoelastic finite element model. The estimated shear modulus decayed nonlinearly over 10 min in each anatomical region, and the range of instantaneous to equilibrium shear modulus was 3.8 to 0.54 kPa in the cerebral cortex, 1.4 to 0.27 kPa in the hippocampus and 1.0 to 0.17 kPa in the caudate/putamen. Although these regions are all gray matter structures, their measured mechanical properties were significantly different. Accurate measurement of inter-regional variations in mechanical properties will contribute to improved understanding organ-level structural parameters and regional differential susceptibility to deformation injury within CNS tissues.
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影响因子：
4.8
作者：
NAGASHIMA, T;TAMAKI, N;SEGUCHI, Y
通讯作者：
SEGUCHI, Y
影响因子：
2.4
作者：
Kaster, T.;Sack, I.;Samani, A.
通讯作者：
Samani, A.
影响因子：
2.2
作者：
Darvish, KK;Crandall, JR
通讯作者：
Crandall, JR
影响因子：
2.4
作者：
Miller, K;Chinzei, K
通讯作者：
Chinzei, K
DOI：
10.1002/jbm.b.31789
发表时间：
2011-04-01
影响因子：
3.4
作者：
Lee, S. J.;Sun, J.;Sarntinoranont, M.
通讯作者：
Sarntinoranont, M.