Environmental DNA (eDNA) Detection Probability Is Influenced by Seasonal Activity of Organisms.
Environmental DNA (eDNA) Detection Probability Is Influenced by Seasonal Activity of Organisms.
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DOI：
10.1371/journal.pone.0165273
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发表时间：
2016
期刊：
影响因子：
3.7
通讯作者：
Larson E
中科院分区：
文献类型：
--
作者：
de Souza LS;Godwin JC;Renshaw MA;Larson E
环境DNA （Environmental DNA, eDNA）在保护应用方面有着巨大的前景，比如监测入侵或濒危物种，但这项新兴技术需要不断的测试，以确定它在何种环境下有效。例如，迄今为止很少有研究评估生物体行为或活动的季节性如何影响eDNA的检测概率。我们应用eDNA对美国阿拉巴马州黑勇士河上游特有的两种高度濒危物种：黑勇士水犬（Necturus alabamensis）和扁麝龟（Sternotherus depressus）进行了调查。重要的是，这些物种具有不同的季节活动模式，其中阿拉巴马白刺在凉爽季节（10 - 4月）更活跃，而抑郁白刺在温暖季节（5 - 9月）更活跃。我们用复制的eDNA水样在两年多的冷暖季节调查了这些物种历史上占据的地点，并在实验室使用物种特异性定量PCR （qPCR）分析。然后利用占用率估计和检测概率模型来评估景观属性对生物存在的影响以及采样季节对eDNA检测概率的影响。重要的是，我们发现季节对两种物种的eDNA检测概率都有很大的影响，阿拉巴马白檀在寒冷季节的eDNA检测概率更高，而抑郁白檀在温暖季节的eDNA检测概率更高。这些结果说明了生物行为或活动对环境中eDNA检测的影响，并确定了基础自然史在设计eDNA监测程序中的重要作用。
Environmental DNA (eDNA) holds great promise for conservation applications like the monitoring of invasive or imperiled species, yet this emerging technique requires ongoing testing in order to determine the contexts over which it is effective. For example, little research to date has evaluated how seasonality of organism behavior or activity may influence detection probability of eDNA. We applied eDNA to survey for two highly imperiled species endemic to the upper Black Warrior River basin in Alabama, US: the Black Warrior Waterdog (Necturus alabamensis) and the Flattened Musk Turtle (Sternotherus depressus). Importantly, these species have contrasting patterns of seasonal activity, with N. alabamensis more active in the cool season (October-April) and S. depressus more active in the warm season (May-September). We surveyed sites historically occupied by these species across cool and warm seasons over two years with replicated eDNA water samples, which were analyzed in the laboratory using species-specific quantitative PCR (qPCR) assays. We then used occupancy estimation with detection probability modeling to evaluate both the effects of landscape attributes on organism presence and season of sampling on detection probability of eDNA. Importantly, we found that season strongly affected eDNA detection probability for both species, with N. alabamensis having higher eDNA detection probabilities during the cool season and S. depressus have higher eDNA detection probabilities during the warm season. These results illustrate the influence of organismal behavior or activity on eDNA detection in the environment and identify an important role for basic natural history in designing eDNA monitoring programs.
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