Searching for resistance genes to Bursaphelenchus xylophilus using high throughput screening.
Searching for resistance genes to Bursaphelenchus xylophilus using high throughput screening.
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DOI：
10.1186/1471-2164-13-599
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发表时间：
2012-11-07
期刊：
影响因子：
4.4
通讯作者：
Vasconcelos MW
中科院分区：
文献类型：
--
作者：
Santos CS;Pinheiro M;Silva AI;Egas C;Vasconcelos MW
松材线虫病是由松材线虫引起的松树枯萎病，在世界范围内造成严重的经济损失。虽然侵染的生态机制已被很好地描述，但植物对病原体的分子反应尚不清楚。这主要是由于缺乏基因组信息和疾病的复杂性。高通量测序是目前检测非模式生物中基因表达的有效方法，因此尽管缺乏基因组序列，但仍提供有价值的信息。在试图解开基因可能参与松树防御对病原体，我们在此报告的高通量比较序列分析的感染和非感染的松树pinaster（非常敏感的PWN）和松树pinnus（不太敏感的PWN）的茎。在完整的454 GS FLX运行中对来自P. pinaster和P. pinaster的感染和未感染茎的四个cDNA文库进行测序，产生总共2，083，698个读数。根据Gene Ontology对组装的转录本编码的推定氨基酸序列进行注释，以将Pinus重叠群分配到生物过程、细胞组分和分子功能类别。大多数注释的转录本对应于云杉属基因-25.4- 39.7%，而较小的百分比，匹配的松属基因，1.8- 12.8%，可能是更多的公共基因组信息的结果云杉比松。比较转录组分析结果表明，苹果酸脱氢酶、阿坝、水分胁迫相关基因和PAR 1在松材线虫侵染后高表达，而蓖麻毒素B相关凝集素、SNARE和高迁移率族基因在松材线虫侵染后高表达。定量PCR实验证实了两种松树之间的差异基因表达。利用454焦磷酸测序技术在松树和松树转录组中检测到由线虫侵染触发的防御相关基因。与转录调控、萜类次生代谢（包括杀线虫活性）和病原菌攻击相关的基因在松杉中的丰度较高。P. pasteuria显示出较高丰度的氧化应激相关基因和较高水平的表达，一般的应激反应基因。本研究提供了关于松树和松树抵抗松材线虫侵染的分子防御机制的重要信息，有助于更好地理解松材线虫病。
Pine wilt disease (PWD), caused by the pinewood nematode (PWN; Bursaphelenchus xylophilus), damages and kills pine trees and is causing serious economic damage worldwide. Although the ecological mechanism of infestation is well described, the plant’s molecular response to the pathogen is not well known. This is due mainly to the lack of genomic information and the complexity of the disease. High throughput sequencing is now an efficient approach for detecting the expression of genes in non-model organisms, thus providing valuable information in spite of the lack of the genome sequence. In an attempt to unravel genes potentially involved in the pine defense against the pathogen, we hereby report the high throughput comparative sequence analysis of infested and non-infested stems of Pinus pinaster (very susceptible to PWN) and Pinus pinea (less susceptible to PWN). Four cDNA libraries from infested and non-infested stems of P. pinaster and P. pinea were sequenced in a full 454 GS FLX run, producing a total of 2,083,698 reads. The putative amino acid sequences encoded by the assembled transcripts were annotated according to Gene Ontology, to assign Pinus contigs into Biological Processes, Cellular Components and Molecular Functions categories. Most of the annotated transcripts corresponded to Picea genes-25.4-39.7%, whereas a smaller percentage, matched Pinus genes, 1.8-12.8%, probably a consequence of more public genomic information available for Picea than for Pinus. The comparative transcriptome analysis showed that when P. pinaster was infested with PWN, the genes malate dehydrogenase, ABA, water deficit stress related genes and PAR1 were highly expressed, while in PWN-infested P. pinea, the highly expressed genes were ricin B-related lectin, and genes belonging to the SNARE and high mobility group families. Quantitative PCR experiments confirmed the differential gene expression between the two pine species. Defense-related genes triggered by nematode infestation were detected in both P. pinaster and P. pinea transcriptomes utilizing 454 pyrosequencing technology. P. pinaster showed higher abundance of genes related to transcriptional regulation, terpenoid secondary metabolism (including some with nematicidal activity) and pathogen attack. P. pinea showed higher abundance of genes related to oxidative stress and higher levels of expression in general of stress responsive genes. This study provides essential information about the molecular defense mechanisms utilized by P. pinaster and P. pinea against PWN infestation and contributes to a better understanding of PWD.
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影响因子：
3.8
作者：
Hanawa, F;Yamada, T;Nakashima, T
通讯作者：
Nakashima, T
影响因子：
4.8
作者：
Ho, CL;Noji, M;Saito, K
通讯作者：
Saito, K
影响因子：
4.4
作者：
Fernández-Pozo N;Canales J;Guerrero-Fernández D;Villalobos DP;Díaz-Moreno SM;Bautista R;Flores-Monterroso A;Guevara MÁ;Perdiguero P;Collada C;Cervera MT;Soto A;Ordás R;Cantón FR;Avila C;Cánovas FM;Claros MG
通讯作者：
Claros MG
影响因子：
14.9
作者：
Apweiler, R;Biswas, W;Zdobnov, E
通讯作者：
Zdobnov, E
影响因子：
4.3
作者：
Damian Daurelio, Lucas;Petrocelli, Silvana;Graciela Orellano, Elena
通讯作者：
Graciela Orellano, Elena