Changes in cell morphology due to plasma membrane wounding by acoustic cavitation.
Changes in cell morphology due to plasma membrane wounding by acoustic cavitation.
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DOI：
10.1016/j.ultrasmedbio.2010.01.010
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发表时间：
2010-04
影响因子：
2.9
通讯作者：
Prausnitz, Mark R.
中科院分区：
文献类型：
--
作者：
Schlicher, Robyn K.;Hutcheson, Joshua D.;Radhakrishna, Harish;Apkarian, Robert P.;Prausnitz, Mark R.
Acoustic cavitation-mediated wounding (i.e., sonoporation) has great potential to improve medical and laboratory applications requiring intracellular uptake of exogenous molecules; however, the field lacks detailed understanding of cavitation-induced morphological changes in cells and their relative importance. Here, we present an in-depth study of the effects of acoustic cavitation on cells using electron and confocal microscopy coupled with quantitative flow cytometry. High resolution images of treated cells show that morphologically different types of blebs can occur after wounding conditions caused by ultrasound exposure as well as by mechanical shear and strong laser ablation. In addition, these treatments caused wound-induced non-lytic necrotic death resulting in cell bodies we call wound-derived perikarya (WD-P). However, only cells exposed to acoustic cavitation experienced ejection of intact nuclei and nearly instant lytic necrosis. Quantitative analysis by flow cytometry indicates that wound-derived perikarya are the dominant morphology of nonviable cells, except at the strongest wounding conditions, where nuclear ejection accounts for a significant portion of cell death after ultrasound exposure.
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影响因子：
2.4
作者：
Guzmán, HR;Nguyen, DX;Prausnitz, MR
通讯作者：
Prausnitz, MR
影响因子：
1.7
作者：
DUNN, F
通讯作者：
DUNN, F
DOI：
10.1115/1.3138276
发表时间：
1981-01-01
影响因子：
1.7
作者：
DEWEY, CF;BUSSOLARI, SR;DAVIES, PF
通讯作者：
DAVIES, PF
影响因子：
8.4
作者：
Lauterborn, W.;Kurz, T.;Lindau, O.
通讯作者：
Lindau, O.
影响因子：
3.4
作者：
Canatella, PJ;Karr, JF;Prausnitz, MR
通讯作者：
Prausnitz, MR